Online Journal of Microbiological Research

Journal profile

Online Journal of Microbiological Research (OJMR) is an international journal dedicated to the latest advancements in Microbiology. The goal of this journal is to provide a platform for scientists and academicians all over the world to promote, share, and discuss various new issues and developments in different areas of Microbiology.

Latest Articles

Open Access September 20, 2022 Endnote/Zotero/Mendeley (RIS) BibTeX

Drug-Receptor Interaction of Peptidic HIV-1 Protease: Polar Effect-II

Online Journal of Microbiological Research 2022, 2(1), 1-11. DOI: 10.31586/ojmr.2022.414
Abstract
Klopman described the chemical reaction of metal ions and base ions in term of softness, En and Em, respectively. By simple modification of known methods, Singh et al. made it applicable for neutral Lewis acids (transition metal salts) and bases (organic molecules) and also extended its application to biological systems
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Klopman described the chemical reaction of metal ions and base ions in term of softness, En and Em, respectively. By simple modification of known methods, Singh et al. made it applicable for neutral Lewis acids (transition metal salts) and bases (organic molecules) and also extended its application to biological systems for site selectivity and to explain reaction mechanism (markovnikov and anti-markovnikov rule), ligand-receptor interaction of testosterones, estrogens and tetrahydroimidazobenzodiazepinone. In this study effective atomic softness En(eff) and Em(eff), and their change ΔEnm have been used for site selectivity and polar interaction between 51 peptidic HIV-1 protease inhibitors and receptor amino acids. ΔEnm values derived from drug-receptor interaction show that when one moiety on receptor behaves as nucleophile (O of valine amino acid) at the same time maximum electrophilic site of the drug (C-atom of the maximum En(eff) value) orient itself to come close the respective site and make maximum interaction, while when another moiety on receptor behaves as electrophilic site (C of isoleucine amino acid), at the same time maximum nucleophilic site of the drug (O-atom of the maximum Em(eff) value) also orient itself to come close the respective site and make maximum interaction.Full article
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Open Access September 4, 2022 Endnote/Zotero/Mendeley (RIS) BibTeX

Drug-Receptor Interaction of Peptidic HIV-1 Protease: The Hydrophobic Effect-I

Online Journal of Microbiological Research 2022, 1(1), 33-48. DOI: 10.31586/ojmr.2022.411
Abstract
When a drug interacts with its receptor, the nonpolar substituent of drug and receptor proteins attract each other because they have opposite magnitude with respect to each other. X-rays structure studies reflected that the S2/S2’ pocket in HIV-1 protease enzyme are essentially hydrophobic. The residues that make up these pockets
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When a drug interacts with its receptor, the nonpolar substituent of drug and receptor proteins attract each other because they have opposite magnitude with respect to each other. X-rays structure studies reflected that the S2/S2’ pocket in HIV-1 protease enzyme are essentially hydrophobic. The residues that make up these pockets are Val-32, Ile-47, Ile-50, and Ile-84 in each monomeric polypeptidic unit of the protease enzyme. Δπdr and ΔSASAdr have been used to measure the extent of hydrophobic interaction between peptidic protease inhibitors and receptor proteins (binding site: valine‒isoleucine; and catalytic site: glycine‒aspartic acid‒threonine) on the HIV-1 protease enzyme. For measurement of hydrophobic interaction, the molecular modeling and geometry optimization of all the inhibitors and the receptor amino acids have been carried out with CAChe Pro software by opting semiempirical PM3 methods. Log P was calculated using the atom-typing scheme of Ghose and Crippen, while solvent accessible surface area by conductor likes screening model. πd, πr, SASASd and SASASr well describe the hydrophobicities of the substituents and play the effective role for site selectivity for interaction of the drug with the receptor. Comparative study of values of Δπdr and ΔSASAdr show the order of hydrophobic interaction with respect to amino acids: Asp > Thr > Val > Ile and Thr > Val > Asp > Ile, respectively. Further, comparative study of the values of (ΣΔπdr)binding-site, (ΣΔπdr)catalytic-site, (ΣΔSASAdr)binding-site, (ΣΔSASAdr)catalytic-site shows that peptidic HIV-1-PRIs interact with binding site rather than catalytic site as binding site have lower value of ΣΔπdr and ΣΔSASAdr. Among the binding site, Val has maximum interaction than Ile, as it has lower vale of Δπdr and ΔSASAdr.Full article
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Open Access August 27, 2022 Endnote/Zotero/Mendeley (RIS) BibTeX

Green Synthesis of Silver Nanoparticles from Various Medicinal Plants

Online Journal of Microbiological Research 2022, 1(1), 25-32. DOI: 10.31586/ojmr.2022.399
Abstract
Chemical solvents are commonly used to prevent microbial growth; dangerous to human health and have limited antibacterial properties. On the other hand, Nanoparticles made of metallic elements (such as copper, silver, and gold) have several uses in the field of biotechnology. Silver nanoparticles are more efficient in their antimicrobial, antibacterial,
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Chemical solvents are commonly used to prevent microbial growth; dangerous to human health and have limited antibacterial properties. On the other hand, Nanoparticles made of metallic elements (such as copper, silver, and gold) have several uses in the field of biotechnology. Silver nanoparticles are more efficient in their antimicrobial, antibacterial, anti-inflammatory and anti-cancer properties. The current study aimed to determine the green synthesis of silver nanoparticles and their antibacterial activity from the aqueous extracts of leaves of Couroupita guianensis, Punica granatum, Vitex negundo, Cirtrus maxima. AgNPs of plant extracts were prepared using silver nitrate with the respective plant extract. Then they were characterized by FTIR analysis. The respective functional groups in the synthesized silver nanoparticles were confirmed with FTIR Spectra. The antibacterial activities of the synthesized nanoparticle extract were observed by zone of inhibition. From the results, the nanoparticles synthesized from the plants extract could pave a way to formulate a drug to treat microbial infection.Full article
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Open Access July 22, 2022 Endnote/Zotero/Mendeley (RIS) BibTeX

DFT-Based Prediction of Anti-Leishmanial Activity of Carboxylates and Their Antimony(III) Complexes Against Five Leishmanial Strains

Online Journal of Microbiological Research 2022, 1(1), 17-24. DOI: 10.31586/ojmr.2022.360
Abstract
Carboxylates and their antimony(III) complexes experimentally scanned earlier for anti-leishmanial activity (IC50) against five leishmanial strains viz., L. major, L. major (Pak), L. tropica, L. mex mex, and L. donovani. These activities have been theoretically predicted by DFT method along with quantitative structure-activity relationship (QSAR) study. Molecular modeling and geometry
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Carboxylates and their antimony(III) complexes experimentally scanned earlier for anti-leishmanial activity (IC50) against five leishmanial strains viz., L. major, L. major (Pak), L. tropica, L. mex mex, and L. donovani. These activities have been theoretically predicted by DFT method along with quantitative structure-activity relationship (QSAR) study. Molecular modeling and geometry optimization of the all the eight compounds have been performed on workspace program of CAChe Pro software of Fujitsu by opting B88-PW91 (Becke '88; Perdew & Wang '91) GGA (generalized-gradient approximation) energy functional with DZVP (double-zeta valence polarized ) basis set in DFT (Density Functional Theory). For QSAR, multiple linear regression (MLR) analysis has been performed on Project Leader Program associated with CAChe. The reliability of correlation between experimental activities and predicted activities are r2 = 0.826, r2CV = 0.426 (L. major); r2 = 0.905, r2CV = 0.507 (L. major (Pak)); r2 = 0.980, r2CV = 0.932 (L. tropica); r2 = 0.781, r2CV = 0.580 (L. mex mex) and r2 = 0.634, r2CV = 0.376 (L. donovani), and a comparison of the experimental values and the values obtained by theoretical calculations has been presented pictorially that shows close resemblance.Full article
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Open Access June 5, 2022 Endnote/Zotero/Mendeley (RIS) BibTeX

Learners’ Perceptions of Computer-Assisted Instruction Approach Teaching and Learning of photosynthesis in Biology Lessons

Online Journal of Microbiological Research 2022, 1(1), 8-16. DOI: 10.31586/ojmr.2022.324
Abstract
The purpose of the study was to investigate the effect of computer-assisted instruction approach to the teaching and learning of photosynthesis on the performance of second year Senior High School (SHS 2) Biology students in science. The design for the study was a quasi-experimental research. This study was carried out
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The purpose of the study was to investigate the effect of computer-assisted instruction approach to the teaching and learning of photosynthesis on the performance of second year Senior High School (SHS 2) Biology students in science. The design for the study was a quasi-experimental research. This study was carried out in Sefwi Wiawso SHS and Asawinso SHS all at Sefwi Wiawso Municipal Assembly in the Western North Region of Ghana. They are all mixed institution. The purposive sampling techniques was used to schools, classes and students for the study. One-hundred one (101) electives biology participants were purposively selected, they consist of SHS 2 Science of (55) fifty-five students from Sefwi Wiawso SHS and SHS 2 Home economics of (46) forty-six students also from Asawinso SHS. The third years were not selected because they were preparing to write their WASSCE. The main instrument for data collection was questionnaire. The study collected only quantitative data and employed quantitative method of data analysis. Data obtained from participants in both experimental and control groups on the Test 2 were analysed statistically using independent-measures t-test. The independent-measures t-Test was used to investigate whether any differences existed between experimental and control groups’ mean scores on the Test 2. The study further revealed that computer-assisted instructions gives feedback to learners to have the opportunity to master computer-assisted instructional package tool used. It is recommended that, computer-assisted instruction method should be encouraged in many Biology classes in Wiawso Municipal Assembly, since it gives students opportunity to see links between concepts, summarise and organise their works, thoughts logically and sequentially. Both genders must be encouraged to use computer-assisted instruction method to studying Biology.Full article
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Open Access March 11, 2022 Endnote/Zotero/Mendeley (RIS) BibTeX

Assessment of Microbiological Quality of Ready to Eat Food Served in Ships Along Warri, Koko and Port Harcourt Water Ways, Nigeria

Online Journal of Microbiological Research 2022, 1(1), 1-7. DOI: 10.31586/ojmr.2021.230
Abstract
Background: Food borne outbreaks have been associated with sourcing unsafe food. Therefore, the first preventative strategy should be to source safe food. Even if the sourced food is safe, measures need to be put in place to ensure that it remains safe during the transfer, storage, preparation and serving activities
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Background: Food borne outbreaks have been associated with sourcing unsafe food. Therefore, the first preventative strategy should be to source safe food. Even if the sourced food is safe, measures need to be put in place to ensure that it remains safe during the transfer, storage, preparation and serving activities that follow. An understanding of the ship food supply and transfer chain will help to illustrate the points at which the food can become contaminated en route to the point of consumption. Objectives: The study was conducted in selected sea port in the core Niger Delta to assessed the microbiological quality of food served at different ship galley to crew and passengers and compered it to standard. Methods: Samples of food were taken from three (Port Harcourt Area one (PHSP), Warri (WSP) and Koko (KSP)) seaports within the South-South zone for laboratory analysis to uncover food spoilage microorganisms capable of causing disease outbreak among ship which could result to Trans border diseases. Eleven samples of different ready to eat food were collected from the locations, which included cooked rice; fried fish, irish potato porridge, vegetable soup, griki, pepper soup, fried irish potato, salad and bread were collected randomly. The samples were prepared and analyzed using standard procedures. Mean viable counts of aerobic and anaerobic bacteria were determined, ranging from (13×103cfu/g to 78×104 cfu/g) for ready to eat food. Results: Based on the finding KSP I, KSP J and KSP K food samples had the highest bacterial contamination on food while WSP F, WSP G and WSP H food samples had the least with the following isolates Salmonella spp, Nocardia spp, Shigella spp, Listeria spp, Bacillus cereus, Leuconostoc spp, Acinetobacter spp, Acetobacter spp, campylobacter spp, Clostridium spp and Vibrio spp which revealed that the isolates were susceptible to any of these antibiotics Septrin, Chloramphenicol, Gentamycin, Tarvid, Streptomycin, Reflacin, Augumetin, Ceporex, Nalidixic acid, Ampicillin, Ciproflox, Penicillin and Erythromycin. Conclusion: Thus, ships operators and regulatory body are expected to take all practicable measures to ensure that they do not receive unsafe or unsuitable food and maintain adequate food temperature at all time.Full article
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ISSN: 2833-9711
DOI prefix: 10.31586/ojmr
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2021-2022
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