Fiber characteristics of Musa balbisiana, Musa paradisiaca and Musa sapientum pseudo-stalks were investigated. Fiber characteristics such as fiber length, fiber diameter, lumen width, and cell wall thickness were measured in the macerated banana pseudo-stalk by microscopy. Slenderness ratio, flexibility coefficient (%) and Runkel ratio were also determined from these microscopic measurements. Mean value of the properties evaluated for Musa sp. respectively were as follows: Fiber length- 2.96mm; Fiber diameter- 21.71µm; Lumen width- 13.55µm; Cell wall thickness- 3.86µm; Flexibility ratio- 66.35%; Runkel ratio- 0.66 and Relative fiber length- 159.12. Based on the findings of this study the Musa sp. pseudo stalk is suitable for pulp and paper production due to its high quality of fibers.