Calotropis procera, a medicinally important plant found in Asia, was explored for its anticancer and antibacterial properties in this study. The leaves of C. procera were extracted using methanol and FTIR and UV-VIS spectrophotometry were used to characterize them. Using the MTT assay and the disc diffusion test, the extract was examined for anticancer activity against the MCF7 breast cancer cell line and antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA). The methanolic fraction of C. procera was found to be efficient against the MCF7 cell line and dramatically suppressed MRSA growth. The metabolic fraction of C. procera leaves is important in suppressing the growth of the MCF7 cell line, and it has the potential to be an effective antibacterial agent, according to our findings. The implications of Calotropis procera for all healthcare professionals including oncologists, physicians, pharmacists, nurses, and nutritional therapists are significant. With the increasing incidence of cancer and antibiotic-resistant bacterial infections, there is a growing need for new, effective, and safer herbal treatments.