@Article{crph166,
AUTHOR = {Iteku, Jeff Bekomo and Bongo, Gédéon Ngiala and Mbiya, Jennifer Ngoie and Wambale, José Mulwahali and Mutwale, Paulin Kapepula and Inkoto, Clément Liyongo and Ngbolua, Koto-te-Nyiwa},
TITLE = {Phytochemical Analysis and Evaluation of Bioactivities of Cola acuminata Extracts},
JOURNAL = {Current Research in Public Health},
VOLUME = {1},
YEAR = {2021},
NUMBER = {1},
PAGES = {51-65},
URL = {/10.31586/jbls-1-1-510.31586/jbls/1/1/5},
ISSN = {2831-5162},
DOI = {10.31586/jbls.2021.166},
ABSTRACT = {Background: From centuries of evolution, knowledge and technological progress for mankind to one day rediscover nature. Currently, the control of bacterial infections is becoming complex due to the concern of antibiotic resistance, which has been a significant global health problem. The aim was to determine and compare phytochemical constituents and in the in vitro evaluation of antimicrobial and antioxidant activities of aqueous, methanol, acetate, dichloromethane extracts from Cola acuminata nuts grown in the Nord Ubangi Province, DRC. Methods: The nuts of Cola acuminata were harvested in April 2016 at Yakoma city, Nord-Ubangi, DRC. The microscopic features of this species were performed in order to identify specific histological structures. Three bacterial strains notably Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 8739 and Pseudomonas aeroginosa ATCC 9027 were used for the assessment of the antibacterial activity. The qualitative and quantitative phytochemical screening were used for compound identification using different fractions and fractions which presented a good extraction yield was used for further analysis. The antioxidant activity was evaluated using ABTS and DPPH scavenging tests while the antibacterial activity was performed using the diffusion method. Findings: The micrography of C. acuminata revealed the presence of following histological elements of which: fibers, spiral vessels, trichomes, ovoid starch grains, sclerenchyma and the fragments of endosperm. Only the methanol and aqueous extracts presented a good extraction yield. The phytochemistry shows the presence of flavonoids, anthocyanins, terpenes, iridoids and tannins. All fractions showed IC50 values lower than 10 µg/mL in the ABTS test and lower than 100 µg/mL in the DPPH test. The antibacterial activity of this plant was low against the three strains used. Conclusion: Seeing the potency of C. acuminata and different biological activities displayed, further analysis are required in order to identify and purify the active ingredients, to study the toxicity of cell lines in vitro, to perform the in vivo experiments and to test for other activities such as the anti-hypoglycemic and anti-inflammatory.},
}

%0 Journal Article
%A Iteku, Jeff Bekomo
%A Bongo, Gédéon Ngiala
%A Mbiya, Jennifer Ngoie
%A Wambale, José Mulwahali
%A Mutwale, Paulin Kapepula
%A Inkoto, Clément Liyongo
%A Ngbolua, Koto-te-Nyiwa
%D 2021
%J Current Research in Public Health

%@ 2831-5162
%V 1
%N 1
%P 51-65

%T Phytochemical Analysis and Evaluation of Bioactivities of Cola acuminata Extracts
%M doi:10.31586/jbls.2021.166
%U /10.31586/jbls-1-1-510.31586/jbls/1/1/5

TY  - JOUR
AU  - Iteku, Jeff Bekomo
AU  - Bongo, Gédéon Ngiala
AU  - Mbiya, Jennifer Ngoie
AU  - Wambale, José Mulwahali
AU  - Mutwale, Paulin Kapepula
AU  - Inkoto, Clément Liyongo
AU  - Ngbolua, Koto-te-Nyiwa
TI  - Phytochemical Analysis and Evaluation of Bioactivities of Cola acuminata Extracts
T2  - Current Research in Public Health
PY  - 2021
VL  - 1
IS  - 1
SN  - 2831-5162
SP  - 51
EP  - 65
UR  - /10.31586/jbls-1-1-510.31586/jbls/1/1/5
AB  - Background: From centuries of evolution, knowledge and technological progress for mankind to one day rediscover nature. Currently, the control of bacterial infections is becoming complex due to the concern of antibiotic resistance, which has been a significant global health problem. The aim was to determine and compare phytochemical constituents and in the in vitro evaluation of antimicrobial and antioxidant activities of aqueous, methanol, acetate, dichloromethane extracts from Cola acuminata nuts grown in the Nord Ubangi Province, DRC. Methods: The nuts of Cola acuminata were harvested in April 2016 at Yakoma city, Nord-Ubangi, DRC. The microscopic features of this species were performed in order to identify specific histological structures. Three bacterial strains notably Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 8739 and Pseudomonas aeroginosa ATCC 9027 were used for the assessment of the antibacterial activity. The qualitative and quantitative phytochemical screening were used for compound identification using different fractions and fractions which presented a good extraction yield was used for further analysis. The antioxidant activity was evaluated using ABTS and DPPH scavenging tests while the antibacterial activity was performed using the diffusion method. Findings: The micrography of C. acuminata revealed the presence of following histological elements of which: fibers, spiral vessels, trichomes, ovoid starch grains, sclerenchyma and the fragments of endosperm. Only the methanol and aqueous extracts presented a good extraction yield. The phytochemistry shows the presence of flavonoids, anthocyanins, terpenes, iridoids and tannins. All fractions showed IC50 values lower than 10 µg/mL in the ABTS test and lower than 100 µg/mL in the DPPH test. The antibacterial activity of this plant was low against the three strains used. Conclusion: Seeing the potency of C. acuminata and different biological activities displayed, further analysis are required in order to identify and purify the active ingredients, to study the toxicity of cell lines in vitro, to perform the in vivo experiments and to test for other activities such as the anti-hypoglycemic and anti-inflammatory.
DO  - Phytochemical Analysis and Evaluation of Bioactivities of Cola acuminata Extracts
TI  - 10.31586/jbls.2021.166
ER  -