@Article{crph219,
AUTHOR = {Nazareth, Aparecida Marcelino de},
TITLE = {Nicotinic agonists promoted the activation of nicotinic acetylcholine α7 receptors (α7 nAChR) in neurons, but failed to activate these receptors in mouse peritoneal macrophages},
JOURNAL = {Current Research in Public Health},
VOLUME = {1},
YEAR = {2022},
NUMBER = {1},
PAGES = {4-19},
URL = {https://www.scipublications.com/journal/index.php/UJPP/article/view/219},
ISSN = {2831-5162},
DOI = {10.31586/ujpp.2022.219},
ABSTRACT = {Nicotinic acetylcholine receptor (nAChR) of subtypes said "neuronal" are expressed in epithelial and immune system cells and participate in acetylcholine signaling by neural or non-neural pathways. It has been shown in macrophages that activation of type α7 nAChRs inhibits the release of pro-inflammatory cytokines, but the ion channel function has not been recorded in these cells. The objective of this work was to clarify what are the molecular mechanisms of transduction of α7 nAChRs in macrophages. To this end, RAW 264.7 cells, mouse peritoneal macrophages and rat hippocampal neurons were used. Cells were submitted to electrophysiological studies and stimulated with brief applications of the agonists acetylcholine, choline and nicotine, associated or not with the allosteric modulator PNU-120596. Responses to ATP were recorded as a reference. Furthermore, macrophages were submitted to cytokine quantitation. The electrophysiological results showed that macrophages responded to ATP but did not show whole-cell current by stimulation with nicotinic agonists. However, hippocampal neurons stimulated in the same pharmacological conditions of the macrophages showed ionic currents typical of the α7 nicotinic receptors. No effect of nicotine was observed in the lipopolysaccharide-induced TNF-α release. These results suggest that the α7 nAChR in macrophages do not work as ion channels similar to those expressed in neurons.},
}

%0 Journal Article
%A Nazareth, Aparecida Marcelino de
%D 2022
%J Current Research in Public Health

%@ 2831-5162
%V 1
%N 1
%P 4-19

%T Nicotinic agonists promoted the activation of nicotinic acetylcholine α7 receptors (α7 nAChR) in neurons, but failed to activate these receptors in mouse peritoneal macrophages
%M doi:10.31586/ujpp.2022.219
%U https://www.scipublications.com/journal/index.php/UJPP/article/view/219

TY  - JOUR
AU  - Nazareth, Aparecida Marcelino de
TI  - Nicotinic agonists promoted the activation of nicotinic acetylcholine α7 receptors (α7 nAChR) in neurons, but failed to activate these receptors in mouse peritoneal macrophages
T2  - Current Research in Public Health
PY  - 2022
VL  - 1
IS  - 1
SN  - 2831-5162
SP  - 4
EP  - 19
UR  - https://www.scipublications.com/journal/index.php/UJPP/article/view/219
AB  - Nicotinic acetylcholine receptor (nAChR) of subtypes said "neuronal" are expressed in epithelial and immune system cells and participate in acetylcholine signaling by neural or non-neural pathways. It has been shown in macrophages that activation of type α7 nAChRs inhibits the release of pro-inflammatory cytokines, but the ion channel function has not been recorded in these cells. The objective of this work was to clarify what are the molecular mechanisms of transduction of α7 nAChRs in macrophages. To this end, RAW 264.7 cells, mouse peritoneal macrophages and rat hippocampal neurons were used. Cells were submitted to electrophysiological studies and stimulated with brief applications of the agonists acetylcholine, choline and nicotine, associated or not with the allosteric modulator PNU-120596. Responses to ATP were recorded as a reference. Furthermore, macrophages were submitted to cytokine quantitation. The electrophysiological results showed that macrophages responded to ATP but did not show whole-cell current by stimulation with nicotinic agonists. However, hippocampal neurons stimulated in the same pharmacological conditions of the macrophages showed ionic currents typical of the α7 nicotinic receptors. No effect of nicotine was observed in the lipopolysaccharide-induced TNF-α release. These results suggest that the α7 nAChR in macrophages do not work as ion channels similar to those expressed in neurons.
DO  - Nicotinic agonists promoted the activation of nicotinic acetylcholine α7 receptors (α7 nAChR) in neurons, but failed to activate these receptors in mouse peritoneal macrophages
TI  - 10.31586/ujpp.2022.219
ER  -