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Article Open Access January 01, 2023

Analysis of D- and L- Isomers of (Meth)amphetamine in Human K2EDTA Plasma

Brian Robbins 1, Jacob Perry 2, Mary Long 1 and Rob E. Carpenter 3,*
1
Department of Research, Advanta Genetics, Tyler, Texas USA
2
Department of Medicine, University of Miami, Miami, Florida, USA
3
Department of Human Resource Development, University of Texas at Tyler, Tyler, Texas, USA
Publihed in: Current Research in Public Health (Volume 3, Issue 1, 2023)
Page(s): 1-12
DOI: 10.31586/jbls.2023.534
Received
October 19, 2022
Revised
December 11, 2022
Accepted
December 29, 2022
Published
January 01, 2023
Keywords
Plasma; Mass Spectrometry; Laboratory Developed Test; Liquid-Liquid Extraction; Chiral Chromatography; Drugs of Abuse; Toxicology
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright: Copyright © The Author(s), 2023. Published by Scientific Publications
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Cite This Article

APA Style
Robbins, B. , Robbins, B. Perry, J. , Perry, J. Long, M. , & Long, M. (2023). Analysis of D- and L- Isomers of (Meth)amphetamine in Human K2EDTA Plasma. Current Research in Public Health, 3(1), 1-12. https://doi.org/10.31586/jbls.2023.534
ACS Style
Robbins, B. ; Robbins, B. Perry, J. ; Perry, J. Long, M. ; Long, M. Analysis of D- and L- Isomers of (Meth)amphetamine in Human K2EDTA Plasma. Current Research in Public Health 2023 3(1), 1-12. https://doi.org/10.31586/jbls.2023.534
Chicago/Turabian Style
Robbins, Brian, Brian Robbins. Jacob Perry, Jacob Perry. Mary Long, and Mary Long. 2023. "Analysis of D- and L- Isomers of (Meth)amphetamine in Human K2EDTA Plasma". Current Research in Public Health 3, no. 1: 1-12. https://doi.org/10.31586/jbls.2023.534
AMA Style
Robbins B, Robbins BPerry J, Perry JLong M, Long M. Analysis of D- and L- Isomers of (Meth)amphetamine in Human K2EDTA Plasma. Current Research in Public Health. 2023; 3(1):1-12. https://doi.org/10.31586/jbls.2023.534 
@Article{crph534,
AUTHOR = {Robbins, Brian and Perry, Jacob and Long, Mary and Carpenter, Rob E.},
TITLE = {Analysis of D- and L- Isomers of (Meth)amphetamine in Human K2EDTA Plasma},
JOURNAL = {Current Research in Public Health},
VOLUME = {3},
YEAR = {2023},
NUMBER = {1},
PAGES = {1-12},
URL = {https://www.scipublications.com/journal/index.php/JBLS/article/view/534},
ISSN = {2831-5162},
DOI = {10.31586/jbls.2023.534},
ABSTRACT = {Methamphetamine and its metabolite amphetamine are frequently abused drugs. Whether obtained legally or from clandestine laboratories it is of relevance to determine the chiral makeup of these drugs for investigative purpose. Although urine and oral fluid matrices are commonly offered, less available to independent laboratories are techniques to verify dextro (D-) or levo (L-) (meth)amphetamine from human K2EDTA plasma. This paper outlines the development and validation of a method that includes the addition of internal standard and a two-step liquid-liquid extraction to remove the analytes from human K2EDTA plasma by triple quadrupole mass spectrometry (LC-MS/MS). The assay was validated according to the United States Food and Drug Administration and College of American Pathologists guidelines, including assessment of the following parameters in plasma validation samples: linear range, limit of detection, lower limit of quantitation, matrix effects, inter- and intra-day assay precision and accuracy, carry over, linearity of dilution, matrix effects and stability. The outcome is a validated and reliable method for the determination of D- and L- isomer concentration of meth(amphetamine) human plasma samples that can be easily adopted by independent clinical laboratories.},
}
%0 Journal Article
%A Robbins, Brian
%A Perry, Jacob
%A Long, Mary
%A Carpenter, Rob E.
%D 2023
%J Current Research in Public Health

%@ 2831-5162
%V 3
%N 1
%P 1-12

%T Analysis of D- and L- Isomers of (Meth)amphetamine in Human K2EDTA Plasma
%M doi:10.31586/jbls.2023.534
%U https://www.scipublications.com/journal/index.php/JBLS/article/view/534

TY  - JOUR
AU  - Robbins, Brian
AU  - Perry, Jacob
AU  - Long, Mary
AU  - Carpenter, Rob E.
TI  - Analysis of D- and L- Isomers of (Meth)amphetamine in Human K2EDTA Plasma
T2  - Current Research in Public Health
PY  - 2023
VL  - 3
IS  - 1
SN  - 2831-5162
SP  - 1
EP  - 12
UR  - https://www.scipublications.com/journal/index.php/JBLS/article/view/534
AB  - Methamphetamine and its metabolite amphetamine are frequently abused drugs. Whether obtained legally or from clandestine laboratories it is of relevance to determine the chiral makeup of these drugs for investigative purpose. Although urine and oral fluid matrices are commonly offered, less available to independent laboratories are techniques to verify dextro (D-) or levo (L-) (meth)amphetamine from human K2EDTA plasma. This paper outlines the development and validation of a method that includes the addition of internal standard and a two-step liquid-liquid extraction to remove the analytes from human K2EDTA plasma by triple quadrupole mass spectrometry (LC-MS/MS). The assay was validated according to the United States Food and Drug Administration and College of American Pathologists guidelines, including assessment of the following parameters in plasma validation samples: linear range, limit of detection, lower limit of quantitation, matrix effects, inter- and intra-day assay precision and accuracy, carry over, linearity of dilution, matrix effects and stability. The outcome is a validated and reliable method for the determination of D- and L- isomer concentration of meth(amphetamine) human plasma samples that can be easily adopted by independent clinical laboratories.
DO  - Analysis of D- and L- Isomers of (Meth)amphetamine in Human K2EDTA Plasma
TI  - 10.31586/jbls.2023.534
ER  -