APA Style
Robbins, B. , Robbins, B. Carpenter, R. E. , Carpenter, R. E. Long, M. , & Long, M. (2023). Quantifying 64 drugs, illicit substances, and D- and L- isomers in human oral fluid with liquid-liquid extraction.
Current Research in Public Health, 3(1), 1-38.
https://doi.org/10.31586/ojms.2023.591
ACS Style
Robbins, B. ; Robbins, B. Carpenter, R. E. ; Carpenter, R. E. Long, M. ; Long, M. Quantifying 64 drugs, illicit substances, and D- and L- isomers in human oral fluid with liquid-liquid extraction.
Current Research in Public Health 2023 3(1), 1-38.
https://doi.org/10.31586/ojms.2023.591
Chicago/Turabian Style
Robbins, Brian, Brian Robbins. Rob E. Carpenter, Rob E. Carpenter. Mary Long, and Mary Long. 2023. "Quantifying 64 drugs, illicit substances, and D- and L- isomers in human oral fluid with liquid-liquid extraction".
Current Research in Public Health 3, no. 1: 1-38.
https://doi.org/10.31586/ojms.2023.591
AMA Style
Robbins B, Robbins BCarpenter RE, Carpenter RELong M, Long M. Quantifying 64 drugs, illicit substances, and D- and L- isomers in human oral fluid with liquid-liquid extraction.
Current Research in Public Health. 2023; 3(1):1-38.
https://doi.org/10.31586/ojms.2023.591
@Article{crph591,
AUTHOR = {Robbins, Brian and Carpenter, Rob E. and Long, Mary and Perry, Jacob},
TITLE = {Quantifying 64 drugs, illicit substances, and D- and L- isomers in human oral fluid with liquid-liquid extraction},
JOURNAL = {Current Research in Public Health},
VOLUME = {3},
YEAR = {2023},
NUMBER = {1},
PAGES = {1-38},
URL = {https://www.scipublications.com/journal/index.php/OJMS/article/view/591},
ISSN = {2831-5162},
DOI = {10.31586/ojms.2023.591},
ABSTRACT = {Although human oral fluid has become more routine for quantitative drug detection in pain management, detecting a large scope of medications and substances is costly and technically challenging for laboratories. This paper presents a quantitative assay for 64 pain medications, illicit substances, and drug metabolites in human oral fluid. The novelty of this assay is that it was developed on an older model AB SCIEX 4000 instrument and renders obscure the need for more technical and expensive laboratory equipment. This method includes addition of internal standard and a 2-step liquid-liquid extraction and dry-down step to concentrate and clean the samples. The samples were suspended in 50% MeOH in water and separation and detection was accomplished using triple quadrupole mass spectrometry (LC-MS/MS). Separation was achieved using reverse-phase liquid chromatography with detection by LC-MS/MS. A second injection was done in negative mode to determine THC-COOH concentration as an indicator of THC. An aliquot of the (already) extracted samples was analyzed for D- and L- isomers of amphetamine and methamphetamine using a chiral column. The standard curve spanned from 5 to 2000 ng/mL for most of the analytes (1 to 2000 ng/mL for fentanyl and THC-COOH) and up to 1000 ng/mL for 13 analytes. Pregabalin and gabapentin ranged from 25 to 2000 ng/mL. The result is a low-cost method for the sensitive detection of a wide-ranging oral fluid menu for pain management. This assay has a high sensitivity, and good precision and accuracy for all analytes with an older model mass spectrometer.},
}
%0 Journal Article
%A Robbins, Brian
%A Carpenter, Rob E.
%A Long, Mary
%A Perry, Jacob
%D 2023
%J Current Research in Public Health
%@ 2831-5162
%V 3
%N 1
%P 1-38
%T Quantifying 64 drugs, illicit substances, and D- and L- isomers in human oral fluid with liquid-liquid extraction
%M doi:10.31586/ojms.2023.591
%U https://www.scipublications.com/journal/index.php/OJMS/article/view/591
TY - JOUR
AU - Robbins, Brian
AU - Carpenter, Rob E.
AU - Long, Mary
AU - Perry, Jacob
TI - Quantifying 64 drugs, illicit substances, and D- and L- isomers in human oral fluid with liquid-liquid extraction
T2 - Current Research in Public Health
PY - 2023
VL - 3
IS - 1
SN - 2831-5162
SP - 1
EP - 38
UR - https://www.scipublications.com/journal/index.php/OJMS/article/view/591
AB - Although human oral fluid has become more routine for quantitative drug detection in pain management, detecting a large scope of medications and substances is costly and technically challenging for laboratories. This paper presents a quantitative assay for 64 pain medications, illicit substances, and drug metabolites in human oral fluid. The novelty of this assay is that it was developed on an older model AB SCIEX 4000 instrument and renders obscure the need for more technical and expensive laboratory equipment. This method includes addition of internal standard and a 2-step liquid-liquid extraction and dry-down step to concentrate and clean the samples. The samples were suspended in 50% MeOH in water and separation and detection was accomplished using triple quadrupole mass spectrometry (LC-MS/MS). Separation was achieved using reverse-phase liquid chromatography with detection by LC-MS/MS. A second injection was done in negative mode to determine THC-COOH concentration as an indicator of THC. An aliquot of the (already) extracted samples was analyzed for D- and L- isomers of amphetamine and methamphetamine using a chiral column. The standard curve spanned from 5 to 2000 ng/mL for most of the analytes (1 to 2000 ng/mL for fentanyl and THC-COOH) and up to 1000 ng/mL for 13 analytes. Pregabalin and gabapentin ranged from 25 to 2000 ng/mL. The result is a low-cost method for the sensitive detection of a wide-ranging oral fluid menu for pain management. This assay has a high sensitivity, and good precision and accuracy for all analytes with an older model mass spectrometer.
DO - Quantifying 64 drugs, illicit substances, and D- and L- isomers in human oral fluid with liquid-liquid extraction
TI - 10.31586/ojms.2023.591
ER -
