Mutational Analysis of Driver and Non-driver Mutations of Philadelphia Chromosome-negative Myeloproliferative Neoplasms; Diagnosis and Recent Advances in Treatment
Table 4.
Methods for MPN mutational analysis.Adapted from: [14].
|
| Method |
Sensitivity (%) |
Advantage |
Disadvantage |
|
|
| Sanger sequencing |
20 |
All mutations detected; bidirectional confirmation |
Poor sensitivity; takes time; not quantitative |
|
| Restriction Fragment Length polymorphism |
1-10 |
Less expensive |
Not quantitative; relatively low sensitivity; requires post-PCR modifications; high + samples required for good results |
|
| Pyrosequencing |
5-10 |
Easy to perform; semiquantitative; less expensive |
Only target mutations detected; relatively low sensitivity |
|
| Melt curve analysis |
5-10 |
Easy to perform; semiquantitative; less expensive |
Only target mutations detected; relatively moderate sensitivity; high + samples required for good results |
|
| Allele-specific PCR |
0.1-1 |
Highly sensitive and easy to perform |
Only target mutations detected; not quantitative |
|
| Locked nuclei acid qPCR |
0.1-0.01 |
Highly sensitive; quantitative |
Only target mutations detected |
|
| Allele-specific qPCR |
0.1-0.01 |
Highly sensitive; quantitative |
Only target mutations detected |
|
|
|
|
