Quantifying 64 drugs, illicit substances, and D- and L- isomers in human oral fluid with liquid-liquid extraction
Table 1.
Statistical analysis for each analyte standard curve over three assays
| Drug / Metabolite | Curve Range (ng/mL) | Mean R | RSD | Mean Slope | SD Slope | N | Fit | | 6-MAM | 5-2000 | 0.9988 | 0.0033 | 0.0340 | 0.0014 | 3.0000 | Quadratic | | 7-Amino Clonazepam | 5-2000 | 0.9991 | 0.0006 | 0.0303 | 0.0129 | 3.0000 | Quadratic | | Alprazolam | 5-2000 | 0.9996 | 0.0002 | 0.0762 | 0.0033 | 3.0000 | Quadratic | | Amitriptyline | 5-1000 | 0.9989 | 0.0005 | 0.0106 | 0.0006 | 3.0000 | Quadratic | | Amphetamine* | 5-1000 | 0.9990 | 0.0006 | 0.0594 | 0.0037 | 3.0000 | Quadratic | | a-OH Alprazolam | 5-2000 | 0.9996 | 0.0003 | 0.0419 | 0.0028 | 3.0000 | Quadratic | | Benzoylecgonine | 5-2000 | 0.9985 | 0.0012 | 0.0018 | 0.0001 | 3.0000 | Quadratic | | Buprenorphine | 5-2000 | 0.9999 | 0.0001 | 0.0241 | 0.0007 | 3.0000 | Quadratic | | Carisoprodol | 5-2000 | 0.9992 | 0.0006 | 0.0098 | 0.0010 | 3.0000 | Quadratic | | Chlordiazepoxide* | 5-1000 | 0.9998 | 0.0000 | 0.0111 | 0.0003 | 3.0000 | Quadratic | | Clonazepam | 5-2000 | 0.9991 | 0.0006 | 0.0161 | 0.0039 | 3.0000 | Quadratic | | Codeine | 5-2000 | 0.9996 | 0.0005 | 0.0406 | 0.0064 | 3.0000 | Quadratic | | Cotinine | 5-2000 | 0.9998 | 0.0001 | 0.0463 | 0.0001 | 3.0000 | Quadratic | | Cyclobenzaprine | 5-2000 | 0.9999 | 0.0001 | 0.0054 | 0.0008 | 3.0000 | Quadratic | | Desalkylflurazepam | 5-2000 | 0.9994 | 0.0001 | 0.0383 | 0.0010 | 3.0000 | Quadratic | | Desipramine | 5-2000 | 0.9993 | 0.0006 | 0.0578 | 0.0047 | 3.0000 | Quadratic | | Diazepam | 5-2000 | 0.9993 | 0.0005 | 0.1009 | 0.0117 | 3.0000 | Quadratic | | Dihydrocodeine | 5-2000 | 0.9986 | 0.0008 | 0.0479 | 0.0028 | 3.0000 | Quadratic | | Doxepin | 5-2000 | 0.9998 | 0.0001 | 0.0211 | 0.0006 | 3.0000 | Quadratic | | EDDP | 5-2000 | 0.9997 | 0.0001 | 0.0106 | 0.0004 | 3.0000 | Quadratic | | Fentanyl | 5-2000 | 0.9998 | 0.0001 | 0.0108 | 0.0006 | 3.0000 | Quadratic | | Fluoxetine | 5-2000 | 0.9995 | 0.0004 | 0.0510 | 0.0028 | 3.0000 | Quadratic | | Gabapentin | 25-2000 | 0.9961 | 0.0033 | 0.0609 | 0.0485 | 3.0000 | Quadratic | | Hydrocodone | 5-2000 | 0.9992 | 0.0005 | 0.0123 | 0.0018 | 3.0000 | Quadratic | | Hydromorphone | 5-2000 | 0.9983 | 0.0021 | 0.1583 | 0.0211 | 3.0000 | Quadratic | | Imipramine | 5-2000 | 0.9999 | 0.0002 | 0.0067 | 0.0007 | 3.0000 | Quadratic | | Ketamine* | 5-1000 | 0.9990 | 0.0009 | 0.0107 | 0.0015 | 3.0000 | Quadratic | | Lorazepam | 5-2000 | 0.9995 | 0.0001 | 0.0091 | 0.0003 | 3.0000 | Quadratic | | MDA* | 5-1000 | 0.9994 | 0.0004 | 0.0550 | 0.0005 | 3.0000 | Quadratic | | MDMA | 5-2000 | 0.9998 | 0.0002 | 0.0050 | 0.0004 | 3.0000 | Quadratic | | MDPV | 5-2000 | 0.9998 | 0.0001 | 0.0777 | 0.0019 | 3.0000 | Quadratic | | Meperidine* | 5-1000 | 0.9995 | 0.0004 | 0.0315 | 0.0020 | 3.0000 | Quadratic | | Meprobamate | 5-2000 | 0.9997 | 0.0003 | 0.0102 | 0.0002 | 3.0000 | Quadratic | | Methadone | 5-2000 | 0.9986 | 0.0006 | 0.0080 | 0.0001 | 3.0000 | Quadratic | | Methamphetamine | 5-2000 | 0.9999 | 0.0001 | 0.0104 | 0.0004 | 3.0000 | Quadratic | | Methylphenidate* | 5-1000 | 0.9993 | 0.0002 | 0.0167 | 0.0007 | 3.0000 | Quadratic | | Morphine | 5-2000 | 0.9988 | 0.0008 | 0.0538 | 0.0038 | 3.0000 | Quadratic | | Naloxone | 5-2000 | 0.9994 | 0.0004 | 0.0548 | 0.0061 | 3.0000 | Quadratic | | Naltrexone | 5-2000 | 0.9991 | 0.0013 | 0.1273 | 0.0067 | 3.0000 | Quadratic | | Nicotine | 5-2000 | 0.9996 | 0.0002 | 0.0712 | 0.0033 | 3.0000 | Quadratic | | Norbuprenorphine | 5-2000 | 0.9997 | 0.0002 | 0.0444 | 0.0026 | 3.0000 | Quadratic | | Nordiazepam | 5-2000 | 0.9991 | 0.0008 | 0.0531 | 0.0061 | 3.0000 | Quadratic | | Norfentanyl | 5-2000 | 0.9996 | 0.0002 | 0.0508 | 0.0057 | 3.0000 | Quadratic | | Norhydrocodone | 5-2000 | 0.9993 | 0.0004 | 0.0858 | 0.0079 | 3.0000 | Quadratic | | Norketamine | 5-2000 | 0.9998 | 0.0001 | 0.0037 | 0.0004 | 3.0000 | Quadratic | | Noroxycodone | 5-2000 | 0.9990 | 0.0005 | 0.0032 | 0.0000 | 3.0000 | Quadratic | | Noroxymorphone | 5-2000 | 0.9998 | 0.0001 | 0.0040 | 0.0002 | 3.0000 | Quadratic | | Nortriptyline | 5-2000 | 0.9983 | 0.0007 | 0.0144 | 0.0006 | 3.0000 | Quadratic | | O-desmethyl Tramadol | 5-2000 | 0.9996 | 0.0002 | 0.0226 | 0.0010 | 3.0000 | Quadratic | | Oxazepam | 5-2000 | 0.9999 | 0.0002 | 0.0249 | 0.0075 | 3.0000 | Quadratic | | Oxycodone | 5-2000 | 0.9992 | 0.0004 | 0.1193 | 0.0112 | 3.0000 | Quadratic | | Oxymorphone | 5-2000 | 0.9989 | 0.0014 | 0.0092 | 0.0006 | 3.0000 | Quadratic | | Paroxetine | 5-2000 | 0.9992 | 0.0003 | 0.0063 | 0.0001 | 3.0000 | Quadratic | | Phencyclidine | 5-2000 | 0.9997 | 0.0001 | 0.0025 | 0.0002 | 3.0000 | Quadratic | | Pregabalin | 25-2000 | 0.9982 | 0.0023 | 0.0784 | 0.0118 | 3.0000 | Quadratic | | Ritalinic Acid* | 5-1000 | 0.9995 | 0.0005 | 0.0094 | 0.0000 | 3.0000 | Quadratic | | Tapentadol | 5-2000 | 0.9994 | 0.0006 | 0.0085 | 0.0006 | 3.0000 | Quadratic | | Temazepam | 5-2000 | 0.9998 | 0.0001 | 0.0189 | 0.0177 | 3.0000 | Quadratic | | Tramadol* | 5-1000 | 0.9994 | 0.0004 | 0.0092 | 0.0010 | 3.0000 | Quadratic | | Venlafaxine | 5-2000 | 0.9996 | 0.0004 | 0.0092 | 0.0010 | 3.0000 | Quadratic | | Zaleplon | 5-2000 | 0.9996 | 0.0004 | 0.0460 | 0.0014 | 3.0000 | Quadratic | | Zolpidem | 5-2000 | 0.9999 | 0.0001 | 0.0100 | 0.0004 | 3.0000 | Quadratic | | Zopiclone | 5-2000 | 0.9991 | 0.0007 | 0.7457 | 1.1989 | 3.0000 | Quadratic | | THC-COOH | 1-2000 | 0.9996 | 0.0004 | 0.0029 | 0.0015 | 3.0000 | Quadratic | | D-Amphetamine | 2.5-1000 | 0.9994 | 0.0003 | 0.0104 | 0.0010 | 3.0000 | Quadratic | | L-Amphetamine | 2.5-1000 | 0.9993 | 0.0006 | 0.0105 | 0.0010 | 3.0000 | Quadratic | | D-Methamphetamine | 2.5-1000 | 0.9995 | 0.0008 | 0.0218 | 0.0021 | 3.0000 | Quadratic | | L-Methamphetamine | 2.5-1000 | 0.9985 | 0.0021 | 0.0246 | 0.0031 | 3.0000 | Quadratic | Note: *Denotes ULOQ of 1000 ng/mL, Gabapentin and Pregabalin had a range from 25 to 2000 ng/mL.
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Note: *Denotes ULOQ of 1000 ng/mL, Gabapentin and Pregabalin had a range from 25 to 2000 ng/mL. |
