Open Journal of Medical Sciences

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Quantifying 64 drugs, illicit substances, and D- and L- isomers in human oral fluid with liquid-liquid extraction

Table 10.

LC-MS/MS conditions for the three assays on a single oral fluid sample.

			P63		THC			D- and L-
Scan type			MRM		MRM			MRM
Ion source			Turbo spray		Turbo spray			Turbo spray
Probe position			X = 5.15, Y = 5.4		X = 5.15, Y = 6.40			X=5.00, Y=5.2
Polarity:			Positive		Negative			Positive
Run duration			8 min		1.5 min			11 min
Settling time (msec)			5		0			0
Pause time (msec)			5		5.0007 msec			7.007 msec
Curtain gas			35		20			35
CAD gas			8		4			4
ISV (V)			5000		-4000			5000
Temperature (°C)			500		400			500
Ion Source Gas 1 (GS1)			50		50			50
Ion Source Gas 2 (GS2)			50		60			50
Q1/Q3 resolution			unit/unit		unit/unit			unit/unit
CEM (V)			2600		2400			2600

Note: multiple reaction monitoring (MRM); collision gas (CAD); ion source voltage (ISV); channel electron multiplier (CEM)

Note: multiple reaction monitoring (MRM); collision gas (CAD); ion source voltage (ISV); channel electron multiplier (CEM)