Inlet Settings

P 63 and THC Panels

D and L Panel

Analytical Column

Restek Ultra Biphenyl 50 x 2.1 mm, 5 µm

Supelco Astek Chirobiotic V 250 x 2.1 mm, 5 µm

Guard Cartridge

Restek Ultra Biphenyl 10 x 4.0 mm, 5 µm

None

Sample Temperature

15 ± 5.0°C

15 ± 5.0°C

Column Temperature

30.0 ± 5.0°C

30.0 ± 5.0°C

Mobile Phase A

Water:MeOH:Formic acid – 97.5:2.5:0.1

Water:Acetic Acid:Ammonium Hydroxide: Methanol 5:1:0.3:993.5

Mobile Phase B

MeOH:Acetonitrile – 1:1

N/A

Needle Rinse

1:1:1

Water:Acetic Acid:Ammonium Hydroxide: Methanol 5:1:0.3:993.5

Flow Rate

0.7 mL/min

0.3 mL/min

Injection Volume

10 μL

10 μL

Run Time

8 min, 1.5 min

11 min