Quantifying 64 drugs, illicit substances, and D- and L- isomers in human oral fluid with liquid-liquid extraction
Table 2.
Inter-assay mean and standard deviation (SD) of validation samples.
| Drug / Metabolite | LLOQ (ng/mL) | LQC (ng/mL) | MQC (ng/mL) | HQC (ng/mL) | | | Mean ± SD | Mean ± SD | Mean ± SD | Mean ± SD | | 6-MAM | 5 ± 0.8 | 14.5 ± 1.9 | 622.3 ± 55.9 | 1928.1 ± 135.3 | | 7-Amino Clonazepam | 5.6 ± 7.89 | 16.7 ± 1.0 | 598..9 ± 29.3 | 1945.9 ± 161.3 | | Alprazolam | 5.0 ± 0.6 | 14.6 ± 1.5 | 573.4 ± 32.5 | 19454 ± 148.8 | | Amitriptyline* | 5.5 ± 0.4 | 16.9 ± 0.8 | 543.9 ± 18.4 | 738.5 ± 40.7 | | Amphetamine* | 5.4 ± 0.5 | 16.7 ± 0.7 | 581.5 ± 24.6 | 802.4 ± 64.4 | | a-OH Alprazolam | 4.8 ± 0.6 | 15.5 ± 1.3 | 595.6 ± 28.6 | 1768.6 ± 118.6 | | Benzoylecgonine | 5.4 ± 0.7 | 16.5 ± 1.1 | 586.7 ± 16.0 | 2069.3 ± 262.1 | | Buprenorphine | 5.0 ± 0.7 | 15.6 ± 0.9 | 588.1 ± 47.3 | 1814.0 ± 172.7 | | Carisoprodol | 5.3 ± 0.6 | 16.1 ± 1.4 | 533.7 ± 34.2 | 1709.8 ± 129.1 | | Chlordiazepoxide* | 5.5 ± 0.7 | 16.5 ± 0.7 | 623.9 ± 34.8 | 778.7 ± 26.3 | | Clonazepam | 5.2 ± 0.9 | 14.5 ± 1.8 | 585.9 ± 49.5 | 1892.9 ± 63.3 | | Codeine | 4.8 ± 0.7 | 16.0 ± 1.7 | 597.7 ± 80.4 | 1932.1 ± 333.6 | | Cotinine | 5.7 ± 0.2 | 15.6 ± 0.5 | 588.5 ± 14.0 | 1883.3 ± 69.5 | | Cyclobenzaprine | 4.8 ± 0.8 | 15.1 ± 1.4 | 591.8 ± 25.6 | 1839.5 ± 88.0 | | Desalkylflurazepam | 4.8 ± 0.5 | 15.8 ± 1.7 | 605.7 ± 54.1 | 1954.7 ± 153.3 | | Desipramine | 5.5 ± 0.3 | 16.0 ± 0.8 | 555.8 ± 38.7 | 1873.5 ± 125.1 | | Diazepam | 5.2 ± 0.3 | 16.2 ± 1.2 | 603.4 ± 45.0 | 2004.6 ± 231.0 | | Dihydrocodeine | 5.1 ± 0.8 | 14.5 ± 1.4 | 527.8 ± 43.0 | 1858.0 ± 170.1 | | Doxepin | 5.1 ± 0.58 | 15.8 ± 0.9 | 587.4 ± 24.3 | 1882.1 ± 74.8 | | EDDP | 4.8 ± 0.3 | 14.6 ± 1.1 | 520.1 ± 26.4 | 1673.9 ± 127.2 | | Fentanyl | 0.9 ± 0.4 | 3.0 ± 0.3 | 568.0 ± 28.5 | 1787.3 ± 91.7 | | Fluoxetine | 5.3 ± 0.6 | 16.2 ± 0.7 | 576.3 ± 34.5 | 1777.0 ± 195.1 | | Gabapentin | 27.8 ± 3.5 | 75.1 ± 12.3 | 603.0 ± 105.7 | 1767.1 ± 206.4 | | Hydrocodone | 5.7 ± 0.8 | 15.9 ± 1.8 | 599.2 ± 61.4 | 1753.3 ± 86.4 | | Hydromorphone | 5.1 ± 0.7 | 14.2 ± 1.6 | 591.5 ± 89.0 | 1873.8 ± 232.1 | | Imipramine | 4.8 ± 0.7 | 15.0 ± 1.1 | 630.2 ± 38.0 | 2040.0 ± 131.8 | | Ketamine* | 5.5 ± 0.4 | 16.5 ± 1.0 | 526.2 ± 49.9 | 734.9 ± 78.5 | | Lorazepam | 5.2 ± 0.8 | 15.8 ± 1.4 | 571.2 ± 25.2 | 1836.7 ± 139.7 | | MDA* | 5.2 ± 0.6 | 16.1 ± 1.3 | 606.6 ± 39.2 | 830.1 ± 41.4 | | MDMA | 5.0 ± 0.4 | 14.4 ± 1.1 | 532.9 ± 40.2 | 1693.1 ± 173.0 | | MDPV | 5.3 ± 0.4 | 15.8 ± 1.2 | 602.1 ± 29.8 | 1894.4 ± 134.5 | | Meperidine* | 5.3 ± 0.3 | 15.9 ± 0.6 | 579.7 ± 23.8 | 773.3 ± 23.6 | | Meprobamate | 5.3 ± 0.5 | 15.6 ± 0.7 | 583.1 ± 23.2 | 1889.6 ± 87.3 | | Methadone | 5.3 ± 0.4 | 17.1 ± 0.5 | 570.2 ± 54.9 | 1685.8 ± 138.8 | | Methamphetamine | 5.1 ± 0.4 | 15.1 ± 0.5 | 585.6 ± 25.9 | 1837 ± 85.7 | | Methylphenidate* | 5.6 ± 0.3 | 16.6 ± 0.7 | 586.0 ± 26.1 | 790.3 ± 26.9 | | Morphine | 5.6 ± 0.6 | 16.6 ± 2.1 | 568.8 ± 67.9 | 1763.8 ± 202.8 | | Naloxone | 5.2 ± 0.6 | 15.4 ± 2.1 | 584.0 ± 48.1 | 1698.5 ± 109.7 | | Naltrexone | 5.2 ± 0.7 | 15.7 ± 1.6 | 566.2 ± 34.8 | 1868.2 ± 172.0 | | Nicotine | 5.0 ± 0.3 | 15.3 ± 0.6 | 592.2 ± 30.0 | 1886.8 ± 150.1 | | Norbuprenorphine | 4.9 ± 0.5 | 15.0 ± 1.3 | 590.8 ± 20.9 | 1814.0 ± 130.7 | | Nordiazepam | 4.8 ± 0.5 | 15.5 ± 1.6 | 571.6 ± 43.9 | 1836.4 ± 122.4 | | Norfentanyl | 5.3 ± 0.5 | 16.4 ± 1.8 | 619.4 ± 50.6 | 1745.5 ± 128.5 | | Norhydrocodone | 5.1 ± 0.6 | 16.1 ± 2.1 | 635.8 ± 58.4 | 1752.7 ± 138.4 | | Norketamine | 5.1 ± 0.4 | 14.6 ± 1.4 | 573.3 ± 37.5 | 1732.5 ± 74.5 | | Noroxycodone | 5.1 ± 0.8 | 16.5 ± 1.4 | 575 ± 76.0 | 1735.3 ± 94.0 | | Noroxymorphone | 5.4 ± 0.7 | 16.2 ± 1.7 | 644.1 ± 45.0 | 1792.5 ± 92.2 | | Nortriptyline | 5.1 ± 0.4 | 15.4 ± 1.7 | 521.5 ± 48.6 | 1656.2 ± 255.8 | | O-desmethyl Tramadol | 5.5 ± 0.4 | 16.2 ± 1.2 | 555.1 ± 33.2 | 1786.9 ± 100.9 | | Oxazepam | 5.4 ± 0.3 | 17.1 ± 0.6 | 583.6 ± 21.6 | 1895.6 ± 53.4 | | Oxycodone | 5.0 ± 0.6 | 14.9 ± 1.4 | 579.5 ± 54.3 | 1722.4 ± 82.0 | | Oxymorphone | 5.1 ± 0.7 | 15.0 ± 1.6 | 580.0 ± 37.5 | 1816.0 ± 84.3 | | Paroxetine | 5.5 ± 0.6 | 16.6 ± 0.9 | 568.4 ± 19.3 | 1842.8160.8 | | Phencyclidine | 4.8 ± 0.6 | 14.5 ± 1.0 | 574.2 ± 24.9 | 1814.1 ± 77.7 | | Pregabalin | 26.2 ± 3.9 | 78.5 ± 12.6 | 571.3 ± 78.8 | 1749.2 ± 188.5 | | Ritalinic Acid* | 5.2 ± 0.9 | 16.0 ± 1.5 | 581.5 ± 58.6 | 782.9 ± 30.5 | | Tapentadol | 5.6 ± 0.4 | 16.1 ± 1.2 | 580.0 ± 28.6 | 1863.8 ± 60.7 | | Temazepam | 5.1 ± 0.8 | 17.2 ± 1.8 | 623.5 ± 29.6 | 1813.8 ± 130.5 | | Tramadol* | 5.1 ± 0.5 | 16.1 ± 0.8 | 572.2 ± 24.3 | 784.7 ± 33.2 | | Venlafaxine | 5.2 ± 0.6 | 16.0 ± 0.4 | 562.3 ± 41.2 | 1782.0 ± 78.7 | | Zaleplon | 5.3 ± 0.4 | 16.4 ± 0.9 | 584.1 ± 34.5 | 1749.2 ± 201.4 | | Zolpidem | 5.0 ± 0.2 | 15.4 ± 0.6 | 574.6 ± 19.6 | 1850.5 ± 91.8 | | Zopiclone | 5.4 ± 0.4 | 15.7 ± 1.5 | 587.5 ± 57.7 | 1815.9 ± 94.2 | | THC-COOH | 0.94 ± 0.12 | 2.94 ± 0.30 | 650.9 ± 65.5 | 1828.2 ± 107.2 | | D-Amphetamine | 2.8 ± 0.1 | 8.3 ± 0.4 | 295.5 ± 14.8 | 940.3 ± 33.4 | | L-Amphetamine | 2.8 ± 0.1 | 8.4 ± 0.3 | 294.5 ± 6.1 | 874.7 ± 25.7 | | D-Methamphetamine | 2.6 ± 0.1 | 7.6 ± 0.3 | 300.6 ± 12.1 | 999.5 ± 48.9 | | L-Methamphetamine | 2.6 ± 0.1 | 7.7 ± 0.4 | 301.6 ± 16.1 | 1013.9 ± 51.4 | Note: Lower limit of quantitation (LLOQ), low quality control (LQC), mid quality control (MQC), high quality control (HQC). Gabapentin and pregabalin had a range from 25 to 2000 ng/mL while the D- and L- assay analytes had a range from 2.5 to 1000 ng/mL.
|
Note: Lower limit of quantitation (LLOQ), low quality control (LQC), mid quality control (MQC), high quality control (HQC). Gabapentin and pregabalin had a range from 25 to 2000 ng/mL while the D- and L- assay analytes had a range from 2.5 to 1000 ng/mL. |
