Quantifying 64 drugs, illicit substances, and D- and L- isomers in human oral fluid with liquid-liquid extraction
Table 3.
Inter-assay precision and accuracy over 3 days with replicates of 6 for a totalof 18 samples.
| Drug / Metabolite | LLOQ | LQC | MQC | HQC | | %CV | %E | %CV | %E | %CV | %E | %CV | %E | | 6-MAM | 16.74 | -0.81 | 13.23 | -3.33 | 8.99 | 3.72 | 7.01 | 7.12 | | 7-Amino Clonazepam | 7.89 | 11.06 | 5.85 | 11.07 | 4.88 | -0.18 | 8.29 | 8.10 | | Alprazolam | 12.90 | -0.43 | 9.98 | -2.78 | 5.67 | -4.43 | 7.65 | 8.05 | | Amitriptyline* | 6.93 | 10.66 | 4.83 | 12.50 | 3.38 | -9.35 | 5.50 | -7.68 | | Amphetamine* | 9.25 | 7.37 | 4.31 | 11.13 | 4.22 | -3.09 | 8.02 | 0.30 | | a-OH Alprazolam | 12.85 | -3.07 | 8.17 | 3.53 | 4.80 | -0.73 | 6.71 | -1.74 | | Benzoylecgonine | 13.11 | 7.70 | 6.47 | 10.27 | 2.72 | -2.22 | 8.48 | 11.00 | | Buprenorphine | 14.36 | -0.4 | 5.91 | 4.00 | 8.03 | -1.98 | 9.52 | 0.78 | | Carisoprodol | 11.91 | 5.21 | 8.89 | 7.49 | 6.41 | -11.05 | 7.55 | -5.01 | | Chlordiazepoxide* | 7.14 | 9.61 | 4.10 | 9.67 | 5.58 | 3.98 | 3.37 | -2.67 | | Clonazepam | 16.14 | 0.00 | 12.17 | -3.44 | 8.45 | -2.36 | 3.34 | 5.16 | | Codeine | 13.99 | -3.39 | 10.35 | 6.82 | 13.45 | -0.38 | 17.27 | 7.34 | | Cotinine | 4.06 | 5.51 | 3.00 | 3.79 | 2.38 | -1.92 | 3.69 | 4.63 | | Cyclobenzaprine | 16.84 | -3.26 | 9.20 | 0.56 | 4.33 | -1.36 | 4.78 | 2.19 | | Desalkylflurazepam | 10.04 | -3.20 | 10.77 | 5.59 | 8.93 | 0.94 | 7.84 | 8.59 | | Desipramine | 6.20 | 9.72 | 5.00 | 6.49 | 6.95 | -7.37 | 6.68 | 4.08 | | Diazepam | 5.41 | 4.07 | 7.13 | 8.04 | 7.46 | 0.56 | 11.53 | 11.37 | | Dihydrocodeine | 11.53 | 11.37 | 9.99 | -3.58 | 8.14 | -12.03 | 9.16 | 3.22 | | Doxepin | 9.43 | 2.88 | 5.60 | 5.47 | 4.14 | -2.10 | 3.97 | 4.56 | | EDDP | 6.33 | -3.09 | 7.20 | -2.42 | 5.08 | -13.31 | 7.60 | -7.01 | | Fentanyl | 20.27 | -5.56 | 8.52 | 0.98 | 5.01 | -5.34 | 5.13 | -0.71 | | Fluoxetine | 11.39 | 6.41 | 4.06 | 7.76 | 5.99 | -3.95 | 10.98 | -1.28 | | Gabapentin | 12.44 | 11.22 | 16.40 | 0.18 | 17.52 | 0.50 | 11.68 | -1.83 | | Hydrocodone | 13.86 | 9.95 | 11.39 | 5.69 | 10.42 | -1.79 | 4.93 | -2.59 | | Hydromorphone | 14.26 | 1.71 | 11.41 | -5.19 | 15.05 | -1.42 | 12.39 | 4.10 | | Imipramine | 15.02 | -3.06 | 7.36 | 0.10 | 6.02 | 5.04 | 6.46 | 13.33 | | Ketamine* | 8.04 | 10.31 | 6.06 | 9.84 | 9.49 | -12.30 | 10.68 | -8.14 | | Lorazepam | 15.07 | 3.43 | 8.96 | 5.01 | 4.41 | -4.81 | 7.55 | 2.04 | | MDA* | 12.07 | 3.29 | 8.28 | 7.08 | 6.47 | 1.10 | 4.98 | 3.76 | | MDMA | 8.48 | -0.69 | 7.68 | -4.10 | 7.55 | -11.18 | 10.22 | -5.94 | | MDPV | 6.69 | 6.29 | 7.75 | 5.64 | 4.94 | 0.34 | 7.10 | 5.24 | | Meperidine* | 5.76 | 6.24 | 4.06 | 5.95 | 4.10 | -3.38 | 3.05 | -3.34 | | Meprobamate | 8.88 | 5.12 | 4.37 | 3.87 | 3.98 | -2.81 | 4.62 | 4.98 | | Methadone | 6.97 | 6.26 | 3.12 | 14.04 | 9.63 | -4.97 | 8.24 | -6.34 | | Methamphetamine | 8.52 | 2.81 | 3.34 | 0.69 | 4.42 | -2.40 | 4.66 | 2.08 | | Methylphenidate* | 5.64 | 12.0 | 4.43 | 10.62 | 4.45 | -2.33 | 2.40 | 4.10 | | Morphine | 12.39 | 4.81 | 12.76 | 10.90 | 11.93 | -5.20 | 11.50 | -2.01 | | Naloxone | 12.47 | 3.68 | 13.40 | 2.80 | 8.23 | -2.66 | 6.46 | -5.64 | | Naltrexone | 13.76 | 3.22 | 9.93 | 4.58 | 6.15 | -5.64 | 9.21 | 3.79 | | Nicotine | 6.21 | -0.71 | 4.17 | 1.96 | 5.06 | -1.31 | 7.95 | 4.82 | | Norbuprenorphine | 11.01 | -2.41 | 8.94 | -0.29 | 3.54 | -1.53 | 7.20 | 0.78 | | Nordiazepam | 9.53 | -4.92 | 10.19 | 3.40 | 7.68 | -4.74 | 6.66 | 2.02 | | Norfentanyl | 10.07 | 5.02 | 10.82 | 9.59 | 8.17 | 3.24 | 7.36 | -3.03 | | Norhydrocodone | 10.88 | 2.93 | 12.81 | 7.52 | 9.18 | 5.96 | 7.89 | -2.63 | | Norketamine | 8.65 | 2.39 | 9.55 | -2.93 | 6.54 | -4.45 | 4.30 | -3.75 | | Noroxycodone | 15.63 | 2.94 | 8.64 | 9.88 | 13.21 | -4.16 | 5.41 | -3.60 | | Noroxymorphone | 13.80 | 8.37 | 10.32 | 7.91 | 6.99 | 7.35 | 5.15 | -0.42 | | Nortriptyline | 7.56 | 1.37 | 10.83 | 2.39 | 9.32 | -13.09 | 15.45 | -7.99 | | O-desmethyl Tramadol | 7.79 | 10.17 | 7.33 | 8.21 | 5.98 | -7.48 | 5.65 | -0.73 | | Oxazepam | 6.46 | 7.36 | 3.64 | 13.71 | 3.70 | -2.74 | 2.81 | 5.31 | | Oxycodone | 11.93 | 0.18 | 9.15 | -0.84 | 9.38 | -3.41 | 4.76 | -4.31 | | Oxymorphone | 14.01 | 1.31 | 10.89 | 0.12 | 6.47 | -3.34 | 4.64 | 0.89 | | Paroxetine | 11.45 | 9.83 | 5.30 | 10.52 | 3.40 | -5.26 | 8.72 | 2.38 | | Phencyclidine | 11.97 | -3.42 | 6.86 | -3.58 | 4.34 | -4.30 | 4.28 | 0.78 | | Pregabalin | 14.93 | 4.96 | 16.12 | 4.62 | 13.80 | -4.79 | 10.78 | -2.82 | | Ritalinic Acid* | 16.35 | 4.00 | 9.65 | 6.73 | 10.07 | -3.08 | 3.89 | -2.13 | | Tapentadol | 8.40 | 1.72 | 7.34 | 7.14 | 4.93 | -3.34 | 3.26 | 3.55 | | Temazepam | 15.56 | 1.18 | 10.34 | 14.88 | 4.75 | 3.91 | 7.19 | 0.77 | | Tramadol | 9.86 | 2.58 | 5.26 | 7.07 | 4.24 | -4.63 | 4.23 | -1.91 | | Venlafaxine | 11.12 | 3.08 | 2.38 | 6.69 | 7.33 | -6.28 | 4.42 | -1.00 | | Zaleplon | 6.67 | 6.56 | 5.48 | 9.54 | 5.90 | -2.64 | 11.51 | -2.82 | | Zolpidem | 4.43 | 0.89 | 3.62 | 2.95 | 3.41 | -4.24 | 4.96 | 2.80 | | Zopiclone | 6.74 | 8.09 | 9.63 | 4.43 | 9.81 | -2.09 | 5.18 | 0.89 | | THC-COOH | 12.59 | -5.52 | 10.35 | -2.07 | 10.06 | 8.49 | 5.88 | 1.56 | | D-Amphetamine | 3.95 | 13.09 | 4.54 | 10.70 | 1.80 | -1.52 | 3.55 | 4.47 | | L-Amphetamine | 4.12 | 10.00 | 3.04 | 11.52 | 2.07 | -1.85 | 2.94 | -2.81 | | D-Methamphetamine | 4.44 | 3.17 | 3.98 | 1.30 | 4.04 | 0.21 | 4.89 | 11.05 | | L-Methamphetamine | 5.54 | 3.21 | 4.98 | 2.33 | 5.32 | 0.52 | 5.07 | 12.66 | Note: lower limit of quantitation (LLOQ), low quality control (LQC), mid quality control (MQC), high quality control (HQC), percent coefficient of variability(%CV), percent error (%E)
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Note: lower limit of quantitation (LLOQ), low quality control (LQC), mid quality control (MQC), high quality control (HQC), percent coefficient of variability(%CV), percent error (%E) |
