Quantifying 64 drugs, illicit substances, and D- and L- isomers in human oral fluid with liquid-liquid extraction
Table 4.
Intra-assay Precision and Accuracy: Precision and Accuracy over 3 days withreplicates of 6 for each day.
| Drug / Metabolite | LLOQ | LQC | MQC | HQC | | %CV | %E | %CV | %E | %CV | %E | %CV | %E | | MIN | MAX | MIN | MAX | MIN | MAX | MIN | MAX | MIN | MAX | MIN | MAX | MIN | MAX | MIN | MAX | | 6-MAM | 9.01 | 18.07 | -15.64 | 12.17 | 7.28 | 13.63 | -12.10 | 4.12 | 4.86 | 9.74 | -3.09 | 11.41 | 2.58 | 4.71 | -0.05 | 15.12 | | 7-Amino Clonazepam | 4.33 | 9.39 | 6.00 | 14.87 | 5.44 | 6.65 | 8.68 | 12.41 | 3.32 | 6.00 | -3.03 | 2.52 | 3.27 | 6.43 | -1.10 | 15.50 | | Alprazolam | 8.16 | 13.14 | -11.60 | 7.20 | 3.35 | 8.88 | -8.82 | 7.32 | 3.12 | 6.64 | -6.64 | -1.90 | 4.77 | 6.81 | 1.42 | 14.77 | | Amitriptyline* | 3.51 | 11.52 | 9.13 | 11.90 | 4.30 | 5.39 | 9.67 | 14.33 | 2.06 | 3.58 | -11.29 | -7.38 | 2.46 | 6.28 | -10.36 | -3.09 | | Amphetamine* | 2.28 | 10.19 | -2.20 | 13.20 | 2.72 | 5.62 | 9.07 | 12.43 | 2.06 | 5.57 | -5.07 | -0.91 | 3.46 | 8.89 | -5.00 | 6.59 | | a-OH Alprazolam | 8.82 | 16.64 | -5.43 | -1.50 | 6.06 | 9.67 | -1.69 | 6.63 | 3.30 | 5.94 | -1.80 | 0.36 | 3.15 | 7.31 | -6.77 | 1.76 | | Benzoylecgonine | 3.95 | 10.99 | -3.30 | 17.00 | 4.07 | 8.20 | 6.22 | 15.23 | 2.13 | 3.39 | -3.23 | -1.16 | 2.48 | 5.94 | 3.38 | 23.18 | | Buprenorphine | 8.06 | 17.33 | -7.13 | 10.33 | 2.54 | 8.69 | 2.84 | 6.02 | 3.76 | 6.68 | -10.06 | 3.63 | 2.14 | 9.87 | -7.80 | 8.99 | | Carisoprodol | 6.63 | 10.14 | -2.07 | 17.70 | 6.03 | 9.21 | 1.10 | 11.82 | 3.99 | 4.47 | -16.33 | -5.62 | 3.79 | 5.61 | -11.33 | 1.84 | | Chlordiazepoxide* | 3.55 | 10.32 | 8.03 | 10.73 | 2.62 | 3.99 | 5.66 | 11.91 | 2.62 | 3.58 | -2.90 | 7.70 | 1.55 | 3.97 | -4.65 | -0.60 | | Clonazepam | 2.17 | 7.66 | -17.17 | 19.65 | 4.59 | 10.69 | -12.53 | 8.71 | 2.52 | 7.67 | -8.92 | 6.07 | 1.01 | 3.80 | 1.65 | 7.30 | | Codeine | 5.91 | 14.73 | -12.08 | 3.07 | 6.84 | 9.35 | -3.67 | 13.09 | 7.06 | 10.54 | -14.33 | 10.37 | 2.72 | 13.64 | -8.97 | 18.21 | | Cotinine | 1.06 | 3.97 | 0.77 | 9.03 | 0.85 | 2.21 | 0.33 | 6.51 | 1.79 | 3.14 | -2.90 | -0.74 | 2.57 | 3.04 | 1.37 | 7.69 | | Cyclobenzaprine | 10.47 | 19.32 | -15.93 | 5.40 | 5.85 | 8.78 | -5.98 | 6.97 | 2.48 | 3.82 | -4.49 | 2.69 | 2.10 | 3.45 | -3.46 | 6.03 | | Desalkylflurazepam | 5.97 | 8.27 | -8.63 | 6.70 | 5.69 | 13.22 | -3.92 | 12.83 | 4.34 | 6.04 | -8.98 | 7.65 | 2.60 | 5.00 | -1.48 | 15.23 | | Desipramine | 5.16 | 7.14 | 8.00 | 11.50 | 2.18 | 7.35 | 4.69 | 9.53 | 1.28 | 7.21 | -13.39 | -1.22 | 2.75 | 3.75 | -1.82 | 9.98 | | Diazepam | 3.25 | 5.61 | -0.30 | 6.30 | 3.58 | 8.12 | 1.24 | 13.38 | 2.11 | 4.41 | -5.58 | 9.68 | 5.38 | 11.58 | 3.58 | 23.59 | | Dihydrocodeine | 10.04 | 16.88 | -9.40 | 12.63 | 7.98 | 13.09 | -7.00 | -1.78 | 4.84 | 10.52 | -13.49 | -10.99 | 4.55 | 9.20 | -3.55 | 9.85 | | Doxepin | 7.00 | 12.15 | 0.10 | 7.27 | 4.92 | 5.47 | 2.13 | 8.61 | 2.31 | 4.47 | -4.49 | 0.38 | 2.80 | 6.07 | 4.07 | 5.02 | | EDDP | 5.06 | 6.14 | -7.17 | 1.37 | 4.29 | 9.80 | -5.34 | 1.72 | 2.75 | 3.37 | -16.90 | -8.59 | 3.09 | 4.92 | -14.97 | -0.45 | | Fentanyl | 14.53 | 23.15 | -26.75 | 1.67 | 7.03 | 8.83 | -4.89 | 4.33 | 1.46 | 3.85 | -8.80 | -0.24 | 3.00 | 4.70 | -5.22 | 2.49 | | Fluoxetine | 5.24 | 14.21 | 3.50 | 8.33 | 2.79 | 5.22 | 6.56 | 8.46 | 3.20 | 5.05 | -8.42 | 1.90 | 5.71 | 7.03 | -9.96 | 10.59 | | Gabapentin | 4.76 | 17.33 | 4.87 | 19.29 | 6.99 | 13.05 | -15.95 | 13.29 | 6.74 | 12.49 | -16.17 | 19.80 | 6.23 | 11.44 | -11.32 | 6.27 | | Hydrocodone | 10.55 | 17.43 | 5.43 | 14.00 | 5.47 | 11.96 | -1.69 | 17.42 | 4.70 | 5.19 | -13.60 | 7.84 | 1.50 | 6.96 | -4.02 | 0.16 | | Hydromorphone | 8.91 | 16.26 | -5.57 | 12.17 | 10.45 | 12.60 | -6.96 | -2.01 | 4.86 | 18.92 | -11.73 | 11.41 | 2.58 | 13.75 | -2.49 | 15.12 | | Imipramine | 9.76 | 16.92 | -7.60 | 4.20 | 2.18 | 12.02 | -0.97 | 0.73 | 1.41 | 4.14 | -0.82 | 11.93 | 2.37 | 4.76 | 5.43 | 18.80 | | Ketamine* | 4.93 | 7.35 | 1.93 | 15.50 | 6.04 | 6.77 | 8.97 | 10.61 | 3.43 | 4.18 | -18.13 | -1.77 | 3.65 | 5.77 | -19.13 | 1.71 | | Lorazepam | 1.11 | 16.90 | -7.33 | 13.15 | 4.27 | 11.16 | 0.73 | 8.02 | 3.08 | 3.64 | -8.77 | -2.71 | 3.59 | 7.28 | -4.13 | 8.19 | | MDA* | 9.43 | 11.52 | -4.93 | 13.07 | 5.30 | 11.03 | 4.78 | 10.87 | 3.40 | 9.79 | 0.09 | 2.31 | 2.21 | 4.70 | 1.37 | 8.37 | | MDMA | 5.74 | 8.93 | -7.23 | 4.70 | 3.36 | 8.15 | -9.64 | 2.56 | 3.15 | 5.42 | -17.29 | -4.25 | 3.84 | 6.78 | -12.90 | 5.72 | | MDPV | 3.00 | 11.27 | 4.93 | 8.77 | 4.60 | 6.19 | -2.82 | 10.82 | 4.20 | 4.86 | -2.45 | 3.66 | 2.27 | 6.38 | -0.25 | 13.82 | | Meperidine* | 1.20 | 5.32 | -0.20 | 9.53 | 1.82 | 3.58 | 1.72 | 9.02 | 1.48 | 5.68 | -6.50 | -1.04 | 1.21 | 4.51 | -5.16 | -2.42 | | Meprobamate | 3.72 | 10.44 | 1.83 | 10.83 | 2.06 | 6.39 | 1.39 | 5.50 | 2.61 | 4.33 | -5.00 | -1.06 | 2.69 | 4.46 | 2.27 | 9.30 | | Methadone | 5.26 | 8.68 | 2.30 | 8.97 | 1.50 | 3.98 | 11.96 | 16.42 | 2.61 | 9.74 | -13.82 | 2.85 | 3.92 | 4.42 | -12.79 | 0.10 | | Methamphetamine | 4.84 | 9.89 | -2.60 | 7.53 | 1.71 | 4.41 | -0.02 | 1.66 | 1.71 | 2.92 | -6.31 | 2.48 | 2.10 | 4.41 | -2.22 | 6.50 | | Methylphenidate* | 5.07 | 6.05 | 9.07 | 13.60 | 2.18 | 4.86 | 7.54 | 14.90 | 1.70 | 2.49 | -7.45 | 1.52 | 2.30 | 4.58 | -2.83 | 0.27 | | Morphine | 9.36 | 13.19 | 0.30 | 13.63 | 8.05 | 10.54 | -1.54 | 21.87 | 6.05 | 8.83 | -17.22 | 4.53 | 7.02 | 9.10 | -10.99 | 9.15 | | Naloxone | 6.04 | 13.40 | -1.03 | 12.70 | 10.16 | 14.14 | -4.99 | 12.33 | 6.59 | 8.14 | -7.62 | 1.54 | 2.47 | 8.16 | -8.08 | -3.20 | | Naltrexone | 9.68 | 15.15 | -3.23 | 11.84 | 8.31 | 11.20 | -0.04 | 8.54 | 4.11 | 6.59 | -7.70 | -1.60 | 4.79 | 11.07 | -0.44 | 8.19 | | Nicotine | 2.98 | 6.09 | -4.40 | 5.10 | 1.49 | 3.80 | -0.34 | 6.23 | 2.27 | 4.29 | -5.74 | 3.74 | 1.67 | 4.22 | -3.38 | 14.85 | | Norbuprenorphine | 6.96 | 10.42 | -11.27 | 5.90 | 5.11 | 8.76 | -4.94 | 7.59 | 2.29 | 4.50 | -2.15 | -0.41 | 3.79 | 10.56 | -2.01 | 3.85 | | Nordiazepam | 5.19 | 9.95 | -11.03 | -1.33 | 4.88 | 14.06 | -2.36 | 9.71 | 5.30 | 6.07 | -10.87 | 1.56 | 3.79 | 5.86 | -4.57 | 5.71 | | Norfentanyl | 7.81 | 11.01 | 0.43 | 10.50 | 8.97 | 11.84 | 6.40 | 15.21 | 6.26 | 9.54 | -1.92 | 6.44 | 5.62 | 8.23 | -7.19 | -0.09 | | Norhydrocodone | 5.68 | 15.60 | 0.40 | 6.60 | 9.43 | 13.18 | -0.61 | 13.97 | 7.21 | 11.23 | 1.61 | 9.54 | 6.06 | 7.61 | -8.22 | 0.31 | | Norketamine | 6.55 | 8.72 | -3.83 | 6.40 | 6.17 | 9.01 | -9.44 | 5.31 | 3.55 | 4.31 | -9.08 | 2.41 | 2.00 | 4.92 | -6.43 | -0.59 | | Noroxycodone | 14.45 | 16.73 | -2.60 | 7.83 | 4.43 | 13.35 | 8.29 | 12.28 | 5.20 | 16.90 | -13.54 | 5.18 | 2.07 | 6.97 | -7.78 | -1.00 | | Noroxymorphone | 9.38 | 17.85 | 3.73 | 16.33 | 7.28 | 12.74 | 4.22 | 12.24 | 2.88 | 3.33 | 3.52 | 11.98 | 2.55 | 7.08 | -3.14 | 3.01 | | Nortriptyline | 7.12 | 7.15 | -1.83 | 4.57 | 3.51 | 5.52 | -11.40 | 11.44 | 2.75 | 2.84 | -19.93 | -2.58 | 2.62 | 4.18 | -18.75 | 11.08 | | O-desmethyl Tramadol | 3.44 | 9.99 | 6.50 | 13.87 | 2.60 | 9.77 | 3.96 | 12.03 | 5.08 | 6.01 | -10.35 | -3.76 | 5.12 | 6.36 | -1.97 | 0.52 | | Oxazepam | 4.77 | 7.79 | 5.20 | 8.43 | 2.35 | 3.72 | 10.57 | 16.98 | 2.30 | 5.13 | -3.76 | -1.67 | 2.31 | 2.45 | 3.73 | 7.78 | | Oxycodone | 8.27 | 9.76 | -11.23 | 6.23 | 6.54 | 11.45 | -5.59 | 3.09 | 6.01 | 10.16 | -10.54 | 0.54 | 3.27 | 4.99 | -7.15 | -1.19 | | Oxymorphone | 9.91 | 16.05 | -4.40 | 8.20 | 7.73 | 14.01 | -5.56 | 7.19 | 4.31 | 7.44 | -6.06 | -1.90 | 3.63 | 5.86 | -0.68 | 2.38 | | Paroxetine | 7.68 | 16.21 | 8.97 | 11.40 | 2.16 | 6.44 | 8.13 | 12.07 | 2.93 | 3.31 | -7.27 | -3.22 | 4.36 | 10.92 | -3.71 | 7.83 | | Phencyclidine | 6.58 | 15.72 | -9.23 | 4.50 | 4.55 | 7.46 | -8.27 | -1.21 | 2.84 | 3.94 | -8.09 | -1.38 | 3.25 | 4.47 | -0.81 | 3.88 | | Pregabalin | 11.84 | 17.10 | -1.53 | 10.89 | 8.00 | 17.30 | -7.30 | 14.20 | 9.76 | 17.69 | -10.64 | 0.72 | 5.82 | 15.67 | -7.15 | -0.35 | | Ritalinic Acid* | 2.91 | 16.92 | -7.03 | 22.63 | 4.01 | 12.30 | -0.56 | 12.41 | 4.22 | 14.76 | -8.74 | 1.37 | 3.68 | 4.52 | -3.08 | -1.18 | | Tapentadol | 1.94 | 10.80 | -1.73 | 7.03 | 3.82 | 5.42 | -1.72 | 12.80 | 1.62 | 5.14 | -7.71 | -0.41 | 2.72 | 3.87 | 2.32 | 5.17 | | Temazepam | 5.27 | 12.57 | -15.80 | 9.80 | 4.85 | 7.18 | 6.83 | 27.88 | 1.15 | 2.54 | -2.14 | 7.89 | 1.32 | 4.02 | -7.81 | 7.95 | | Tramadol | 3.94 | 8.92 | -5.03 | 11.77 | 4.73 | 5.09 | 4.71 | 10.89 | 2.47 | 4.92 | -6.52 | -3.37 | 3.58 | 5.70 | -3.08 | -1.10 | | Venlafaxine | 5.86 | 7.08 | -10.10 | 10.27 | 1.70 | 2.82 | 5.80 | 8.38 | 3.01 | 5.73 | -14.40 | -1.74 | 1.86 | 2.84 | -5.99 | -0.86 | | Zaleplon | 4.79 | 6.74 | 1.67 | 9.77 | 0.91 | 6.98 | 5.97 | 13.88 | 4.74 | 6.08 | -5.49 | 1.63 | 4.94 | 14.24 | -13.41 | 5.24 | | Zolpidem | 2.24 | 5.12 | -1.43 | 4.43 | 2.48 | 3.48 | 0.82 | 6.28 | 1.08 | 2.64 | -7.17 | -0.79 | 1.26 | 3.41 | -1.48 | 7.09 | | Zopiclone | 4.44 | 9.90 | 6.57 | 10.10 | 7.13 | 8.32 | -3.02 | 12.93 | 3.98 | 7.60 | -13.09 | 6.17 | 2.66 | 3.99 | -3.07 | 4.84 | | THC-COOH | 5.20 | 7.38 | -17.50 | 5.17 | 3.82 | 10.03 | -13.78 | 4.94 | 2.67 | 6.62 | -6.60 | 20.04 | 1.18 | 8.33 | -4.49 | 6.79 | | D-Amphetamine | 1.38 | 3.51 | 8.44 | 15.79 | 1.14 | 1.40 | 4.38 | 15.69 | 0.69 | 1.73 | -2.89 | -0.02 | 1.46 | 2.99 | 0.75 | 7.90 | | L-Amphetamine | 2.60 | 4.47 | 6.63 | 13.33 | 1.11 | 1.79 | 9.38 | 15.71 | 1.14 | 1.57 | -3.13 | 0.31 | 1.46 | 2.33 | -5.16 | 0.02 | | D-Methamphetamine | 1.28 | 4.42 | -1.56 | 5.73 | 1.67 | 2.66 | -3.19 | 4.96 | 1.75 | 2.21 | -4.28 | 4.24 | 1.67 | 2.74 | 7.59 | 17.89 | | L-Methamphetamine | 1.66 | 5.19 | -2.83 | 7.65 | 2.59 | 3.56 | -3.14 | 6.69 | 0.55 | 0.99 | -4.74 | 7.49 | 2.17 | 5.87 | 8.68 | 17.91 | Lower limit of Quantitation (LLOQ), Low Quality Control (LQC), Mid Quality Control (MQC), High Quality Control (HQC), Percent Coefficient of Variability(%CV), Percent error (%E)
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Lower limit of Quantitation (LLOQ), Low Quality Control (LQC), Mid Quality Control (MQC), High Quality Control (HQC), Percent Coefficient of Variability(%CV), Percent error (%E) |