Quantifying 64 drugs, illicit substances, and D- and L- isomers in human oral fluid with liquid-liquid extraction
Table 6.
Stability testing
| Drug / Metabolite | F/T 3 Cycles | Overnight Stability | Post Preparation Stability | | QC | %Diff | RT | 4 °C | -20 °C | Init % Diff Nom | %Diff Init Day 3 | %Diff Init Day 7 | | 6-MAM | QC 75 | -2.20 | 0.37% | 9.21% | 5.02% | -6.42 | 7.33 | -4.32 | | | QC 1800 | -0.27 | 0.45% | -4.49% | 3.41% | -0.05 | -3.14 | 1.85 | | 7-Amino Clonazepam | QC 75 | 10.66 | 2.28% | 17.26% | 7.13% | 8.93 | -3.36 | 2.09 | | ONS 800 | QC 1800 | -4.90 | 12.65% | 9.31% | 6.37% | 9.92 | -11.74 | -5.05 | | Alprazolam | QC 75 | -1.00 | 4.68% | 2.81% | -1.21% | -3.41 | 7.16 | 14.54 | | | QC 1800 | -0.20 | 2.58% | 1.50% | -1.41% | 7.96 | -4.16 | -4.08 | | Amitriptyline* | QC 75 | -2.04 | 0.22% | 3.61% | 1.96% | 1.00 | 12.62 | 1.45 | | | QC 800 | 9.65 | 4.13% | 2.63% | 1.50% | -9.60 | 15.57 | -0.15 | | Amphetamine* | QC 75 | 0.69 | 2.48% | 4.91% | 3.63% | 8.95 | -1.33 | 0.61 | | | QC 800 | 6.92 | 6.21% | 8.19% | 0.45% | -0.69 | 0.16 | -1.78 | | a-OH Alprazolam | QC 75 | -2.90 | 1.92% | -1.67% | 1.06% | 4.34 | 1.24 | 0.61 | | | QC 1800 | 5.65 | -3.26% | -4.27% | -3.07% | -6.77 | 5.40 | -0.97 | | Benzoylecgonine | QC 75 | -1.50 | -2.98% | -3.08% | -4.04% | 10.96 | -7.92 | -5.41 | | | QC 1800 | 2.86 | 14.55% | 7.55% | 6.46% | 3.38 | -5.69 | -2.43 | | Buprenorphine | QC 75 | -0.88 | 1.21% | 3.69% | 4.60% | -0.50 | 5.34 | 8.39 | | | QC 1800 | 6.09 | -2.14% | -7.59% | -3.04% | -7.80 | 9.56 | 2.26 | | Carisoprodol | QC 75 | 5.43 | -4.08% | -2.76% | -5.62% | -12.87 | 19.72 | 19.85 | | PPS 800 | QC 1800 | 19.85 | 4.95% | 1.29% | 1.04% | -15.18 | 24.55 | 4.48 | | Chlordiazepoxide* | QC 75 | -3.47 | -0.48% | 6.45% | 0.91% | 9.20 | 1.17 | 2.75 | | | QC 800 | 3.55 | 0.00% | 9.10% | 0.13% | -4.65 | 7.22 | -1.34 | | Clonazepam | QC 75 | 2.80 | 6.97% | 4.37% | 4.61% | -10.90 | 4.09 | 0.16 | | | QC 1800 | -9.45 | 1.80% | 1.86% | -4.38% | 7.30 | -6.66 | -5.02 | | Codeine | QC 75 | -3.96 | 19.00% | 9.94% | 9.73% | -2.28 | 6.05 | 9.90 | | | QC 1800 | -12.06 | 0.82% | 26.73% | -0.29% | -8.97 | 13.89 | 16.65 | | Cotinine | QC 75 | 1.22 | 0.41% | 1.90% | 2.01% | 4.80 | 0.75 | 0.87 | | | QC 1800 | 5.91 | 5.23% | 5.45% | 4.87% | 1.37 | -2.14 | 1.60 | | Cyclobenzaprine | QC 75 | 2.83 | -6.30% | -5.95% | -3.01% | 0.41 | 6.88 | -1.66 | | | QC 1800 | 1.78 | 2.76% | 0.53% | 1.08% | 4.01 | 3.38 | -6.97 | | Desalkylflurazepam | QC 75 | 6.90 | 17.91% | 12.27% | 13.14% | -3.44 | 4.37 | 2.63 | | | QC 1800 | 3.74 | 9.41% | 10.55% | 8.60% | -1.48 | 2.70 | -1.49 | | Desipramine | QC 75 | 2.04 | -4.28% | -1.62% | -5.69% | -0.55 | 4.57 | -8.48 | | | QC 1800 | 4.20 | 6.72% | 1.40% | 6.24% | -1.82 | -3.83 | 20.30 | | Diazepam | QC 75 | -5.21 | 3.33% | 0.24% | 7.38% | 12.34 | -5.19 | -4.47 | | | QC 1800 | -2.30 | 7.38% | 3.54% | 146.79% | 3.58 | -5.71 | -5.34 | | Dihydrocodeine | QC 75 | 11.06 | -8.33% | -7.02% | -10.91% | -15.70 | 24.73 | 16.45 | | | QC 1800 | 1.34 | -0.50% | 1.96% | -0.62% | -3.55 | -4.30 | 4.95 | | Doxepin | QC 75 | 1.13 | 2.68% | 4.02% | 3.87% | 3.06 | 3.95 | 2.09 | | | QC 1800 | -0.85 | 2.58% | -0.48% | -1.93% | 4.59 | -9.95 | -4.95 | | EDDP | QC 75 | 2.31 | -5.45% | -3.97% | -2.03% | -7.70 | 1.48 | -0.99 | | | QC 1800 | 4.94 | 3.14% | -0.31% | -0.29% | -14.97 | 9.53 | -1.09 | | Fentanyl | QC 75 | -3.57 | -3.38% | -3.48% | -4.17% | 2.08 | 8.06 | -2.08 | | | QC 1800 | 3.18 | -0.51% | -5.38% | -1.50% | 0.61 | 8.51 | -8.58 | | Fluoxetine | QC 75 | 3.23 | 2.31% | -2.96% | 0.26% | 5.54 | -1.51 | 2.24 | | | QC 1800 | 10.50 | -0.17% | 5.65% | 6.69% | -9.96 | 28.08 | 8.37 | | Gabapentin | QC 75 | -10.93 | -1.89% | 2.07% | -4.51% | 3.20 | -4.59 | -10.84 | | | QC 1800 | 11.46 | 6.11% | -1.67% | 1.25% | -11.32 | 24.72 | 18.36 | | Hydrocodone | QC 75 | -12.58 | -3.42% | 9.56% | 5.48% | 8.60 | -7.96 | -9.36 | | | QC 1800 | 4.85 | 1.95% | -0.65% | -2.16% | -3.92 | -0.19 | 0.55 | | Hydromorphone | QC 75 | -9.70 | -7.86% | -13.60% | -16.80% | -5.23 | 9.80 | 12.17 | | | QC 1800 | 10.21 | -11.48% | -16.99% | -12.45% | -2.49 | 11.75 | 7.07 | | Imipramine | QC 75 | 0.96 | -12.08% | -1.44% | -5.54% | 0.20 | 7.62 | -5.36 | | | QC 1800 | 0.28 | 1.75% | -1.34% | -0.13% | 15.77 | -0.19 | 3.35 | | Ketamine* | QC 75 | 2.45 | 2.13% | 7.09% | 1.66% | 19.19 | -9.90 | -1.38 | | | QC 800 | 9.55 | 10.32% | 11.64% | -0.90% | 1.71 | -10.91 | 10.35 | | Lorazepam | QC 75 | -3.48 | -3.38% | -0.67% | -5.00% | -5.55 | 15.01 | 2.82 | | | QC 1800 | -1.28 | -3.58% | -1.38% | 1.20% | 2.06 | 1.72 | -9.89 | | MDA* | QC 75 | 0.06 | 0.27% | 1.81% | 2.03% | 7.62 | -6.71 | 3.25 | | | QC 800 | -4.05 | 3.52% | 1.68% | 2.98% | 1.37 | -11.16 | -5.75 | | MDMA | QC 75 | 0.68 | 0.62% | -1.26% | 8.37% | -6.71 | 3.19 | -5.90 | | | QC 1800 | 4.07 | -0.50% | -0.23% | 0.77% | -14.82 | 5.73 | -3.76 | | MDPV | QC 75 | 4.00 | -0.34% | -3.95% | -1.89% | 4.90 | 0.54 | 4.39 | | | QC 1800 | -1.79 | 7.33% | 0.74% | 4.58% | 2.16 | 8.92 | 0.51 | | Meperidine* | QC 75 | 0.42 | -1.85% | -0.82% | 0.40% | 1.52 | 3.20 | 7.84 | | | QC 800 | -2.89 | 1.79% | 7.09% | 0.91% | -5.16 | -2.62 | 0.02 | | Meprobamate | QC 75 | 1.79 | 1.76% | 2.42% | 1.59% | 5.44 | -0.61 | 1.66 | | | QC 1800 | -2.28 | 5.60% | 1.61% | 4.11% | 3.37 | 2.40 | 1.38 | | Methadone | QC 75 | 2.34 | -1.56% | -0.37% | 3.02% | 7.70 | -9.79 | 4.73 | | PPS 800 | QC 1800 | 6.05 | 3.06% | 4.73% | -0.82% | -14.62 | 5.81 | 5.79 | | Methamphetamine | QC 75 | -2.38 | 1.39% | -0.23% | 0.72% | -1.25 | 2.61 | 6.13 | | | QC 1800 | 1.12 | 2.62% | 1.95% | 4.85% | -2.22 | -4.26 | -1.39 | | Methylphenidate* | QC 75 | -7.10 | -2.61% | -2.11% | -2.06% | 8.53 | -0.92 | -4.95 | | | QC 800 | 2.19 | 5.03% | 5.00% | -0.14% | -1.09 | -2.99 | 2.13 | | Morphine | QC 75 | -0.92 | -5.57% | -1.17% | -4.08% | 2.17 | 4.13 | 10.72 | | | QC 1800 | 3.83 | -3.53% | -4.01% | -4.63% | -4.19 | 8.65 | -0.29 | | Naloxone | QC 75 | -10.27 | 12.63% | 9.79% | 8.83% | 19.07 | -18.32 | -10.78 | | PPS 800 | QC 1800 | -7.54 | 2.11% | -10.26% | -18.08% | -9.13 | 2.71 | 18.19 | | Naltrexone | QC 75 | 5.97 | -2.60% | 4.14% | 0.05% | 3.52 | -4.78 | -6.41 | | ONS 800 | QC 1800 | 10.54 | 8.83% | 7.26% | 2.19% | 8.19 | -0.03 | -8.48 | | Nicotine | QC 75 | 0.39 | 2.91% | 2.15% | 3.93% | 4.95 | -2.80 | -1.63 | | | QC 1800 | 9.43 | -4.80% | -5.13% | -6.90% | -3.38 | 0.68 | -2.68 | | Norbuprenorphine | QC 75 | 3.28 | 3.59% | 2.85% | 7.26% | -1.27 | 2.82 | -4.35 | | | QC 1800 | 3.71 | 0.08% | 1.69% | -1.59% | 3.85 | -11.04 | 8.99 | | Nordiazepam | QC 75 | 2.43 | 1.48% | 5.51% | 13.46% | -3.60 | 0.65 | 2.30 | | | QC 1800 | 6.51 | 7.63% | 9.95% | 2.25% | -4.57 | 5.39 | -5.18 | | Norfentanyl | QC 75 | 2.45 | -0.94% | 6.41% | 3.79% | -1.12 | -4.10 | 2.77 | | | QC 1800 | 9.80 | 9.77% | 3.53% | 14.30% | -7.19 | 18.81 | 12.04 | | Norhydrocodone | QC 75 | 8.20 | 4.74% | 13.09% | 4.93% | -3.36 | -5.99 | 6.15 | | | QC 1800 | 1.72 | 9.52% | 2.23% | 12.80% | -8.22 | 13.41 | 10.19 | | Norketamine | QC 75 | -1.55 | 2.13% | 2.63% | 1.49% | 1.84 | -1.36 | -8.48 | | | QC 1800 | 0.76 | -2.05% | -3.04% | -3.00% | -6.43 | 12.08 | 3.53 | | Noroxycodone | QC 75 | -3.74 | 3.63% | -1.24% | 3.67% | -0.05 | 3.02 | 1.52 | | PPS 800 | QC 1800 | -7.22 | -1.64% | -6.30% | -10.53% | -12.69 | 4.35 | 1.35 | | Noroxymorphone | QC 75 | -4.15 | 2.58% | 7.31% | -1.31% | 7.66 | -5.65 | -8.59 | | | QC 1800 | 6.57 | 2.33% | -2.68% | -3.01% | 3.01 | -0.65 | 0.11 | | Nortriptyline | QC 75 | 1.42 | -2.16% | -2.66% | -3.74% | 3.60 | -2.01 | -1.84 | | ONS 800 | QC 1800 | 2.32 | 0.88% | 4.53% | -1.62% | -18.75 | -1.42 | -1.43 | | O-desmethyl Tramadol | QC 75 | 4.83 | -2.42% | -0.15% | 1.14% | 4.59 | 1.59 | 8.44 | | QC 1800 | -2.24 | -1.57% | -2.02% | -1.32% | -1.97 | -4.59 | 2.44 | | Oxazepam | QC 75 | -0.87 | 1.14% | 1.71% | 0.58% | 10.98 | 4.02 | 1.09 | | | QC 1800 | 2.95 | 6.70% | 8.07% | 0.81% | 4.43 | 0.48 | -0.24 | | Oxycodone | QC 75 | 2.86 | 4.02% | 6.06% | 7.37% | 0.30 | 1.97 | 7.36 | | | QC 1800 | 10.88 | 3.48% | 3.65% | 3.32% | -7.15 | 17.30 | 14.27 | | Oxymorphone | QC 75 | -5.16 | 0.11% | -0.19% | -0.88% | 4.83 | -3.38 | -8.52 | | | QC 1800 | 4.05 | 3.31% | -0.68% | -0.53% | -0.68 | -4.13 | 0.18 | | Paroxetine | QC 75 | -6.32 | -0.17% | -3.40% | 0.80% | 0.46 | 1.92 | -3.09 | | | QC 1800 | 17.77 | 1.19% | 1.98% | 6.18% | -0.81 | 6.60 | -2.20 | | Phencyclidine | QC 75 | -1.78 | -2.81% | -5.88% | -3.94% | -5.13 | 17.54 | 4.33 | | | QC 1800 | -0.06 | 1.95% | 3.49% | 4.00% | 3.88 | 1.68 | -5.21 | | Pregabalin | QC 75 | 2.39 | -12.03% | -9.01% | -9.79% | -7.30 | 8.64 | 17.99 | | | QC 1800 | -14.89 | 21.86% | 4.03% | -2.40% | -0.35 | -15.84 | 0.18 | | Ritalinic Acid* | QC 75 | -1.26 | 0.35% | 5.55% | 4.21% | -0.75 | 6.55 | 2.52 | | | QC 800 | 4.14 | 1.15% | 0.90% | -4.31% | -3.08 | 0.29 | 0.84 | | Tapentadol | QC 75 | -5.00 | -6.63% | -6.52% | -4.14% | 10.77 | 0.03 | -4.42 | | | QC 1800 | 3.61 | 3.54% | -3.63% | 1.82% | 2.32 | 2.64 | 2.38 | | Temazepam | QC 75 | 2.49 | 2.90% | 4.22% | 3.29% | 31.59 | -4.08 | -13.60 | | PPS 800 | QC 1800 | 12.24 | 12.91% | 6.29% | 7.40% | -8.50 | -1.82 | 8.80 | | Tramadol* | QC 75 | -0.90 | 6.65% | 0.75% | 6.01% | 0.94 | 11.42 | 13.30 | | | QC 800 | 6.76 | 0.19% | 6.65% | -3.32% | -1.10 | 2.00 | 1.67 | | Venlafaxine | QC 75 | -2.72 | -0.92% | -0.11% | -0.44% | 12.47 | -3.57 | -0.39 | | PPS 800 | QC 1800 | 10.18 | 3.62% | -0.13% | 1.04% | -3.56 | -6.22 | -0.23 | | Zaleplon | QC 75 | 3.03 | -0.94% | -3.45% | 0.23% | 5.27 | -3.72 | 6.29 | | ONS 800 | QC 1800 | 1.39 | 13.52% | 12.23% | -3.20% | -13.41 | 13.37 | 9.33 | | Zolpidem | QC 75 | -3.32 | -0.55% | 0.25% | -0.28% | 4.41 | 3.17 | 4.68 | | | QC 1800 | -0.55 | 1.58% | 1.37% | -1.22% | -1.48 | 2.41 | 3.07 | | Zopiclone | QC 75 | -3.46 | 0.99% | 6.90% | -0.62% | 7.26 | -8.39 | -4.82 | | | QC 1800 | 14.70 | -2.41% | 0.20% | -0.14% | -3.07 | 0.74 | 4.95 | | THC-COOH | QC 15 | 0.07 | 6.16% | 3.89% | -2.86% | -1.13 | 3.29 | 1.84 | | | QC 1800 | 0.07 | 1.64% | 4.99% | 0.74% | 6.79 | -9.55 | -2.10 | Note: *Indicates high QC of 800; overnight study (ONS), post preparation stability (PPS). QC samples were tested for stability after 3 freeze thaw cycles and tested overnight at the indicated temperatures. Three and seven day post extraction studies were also performed at 2-8 °C
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Note: *Indicates high QC of 800; overnight study (ONS), post preparation stability (PPS). QC samples were tested for stability after 3 freeze thaw cycles and tested overnight at the indicated temperatures. Three and seven day post extraction studies were also performed at 2-8 °C |
