Articles

Silver nanoparticles (Ag<SUP>+</SUP>NPs) contamination in the environment is a serious concern. This study investigated selected heavy metal (Ag<SUP>+</SUP>, Cd<SUP>2+,</SUP> Cr<SUP>2+</SUP> and Pb<SUP>2+</SUP>) concentrations at different sampling points to assess the risk to human health (infants, children, and adults). To do this, an enclosed area (laboratory) of 12.6 m X 8.5 m (107.1 m<SUP>2</SUP>) was clearly marked at different coded distances of S1, S2, S3, and S4 representing 2, 4, 6, and 8 m, while unpolluted atmosphere at 50 m away without Ag<SUP>+</SUP>NPs served as the control (S5). The silver fireworks were allowed to burn for an approximate 00h03m30s at each sampling points using a high-volume air sampler mounted at the Environmental Engineering Departmental Laboratory, Rivers State University, with windows and doors closed to simulate indoor conditions. Samples were digested using a mixture of analytical-grade nitric acid, analytical-grade hydrochloric acid and analyzed to evaluate the levels of heavy metals by atomic absorption spectrophotometry. The Ag<SUP>+</SUP> result at S1 shows 30,000 µg/cm<SUP>3</SUP>, S2 was 29,000 µg/cm<SUP>3</SUP>, while S3 was 28000 µg/cm<SUP>3</SUP> and then S4 was 13,000 µg/cm<SUP>3</SUP>. These results exceeded the permissible values of the United States National Ambient Air Concentration for rural, urban and industrial areas (0.0005, 0.004 and 0.6 µg/cm<SUP>3</SUP>, respectively). The result for the control (S5) (0.037 µg/cm<SUP>3</SUP>) was within the maximum allowable value. Results from other heavy metals such as Cd were 1000, 743, 401, 153, 0.001 µg/cm<SUP>3</SUP>, Cr was 5000, 4000, 3729, 2960, 0.002 µg/cm<SUP>3</SUP>, Pb was 0.048, 0.041, 0.035, 0.034 and 0.01, µg/cm<SUP>3</SUP>, respectively. However, higher values of Ag<SUP>+</SUP>, Cd, and Cr indicated a higher propensity for the metals to be toxic (bioavailable). In addition, the assessment of the potential health risk posed by these metals proved contaminated and harmful. Visitors recorded high values in exposure concentration<b> (</b>EC) and low values in average daily dose (ADD).

Chemical solvents are commonly used to prevent microbial growth; dangerous to human health and have limited antibacterial properties. On the other hand, Nanoparticles made of metallic elements (such as copper, silver, and gold) have several uses in the field of biotechnology<b>. </b>Silver nanoparticles are more efficient in their antimicrobial, antibacterial, anti-inflammatory and anti-cancer properties. The current study aimed to determine the green synthesis of silver nanoparticles and their antibacterial activity from the aqueous extracts of leaves of <i>Couroupita</i><i> </i><i>guianensis</i><i>, </i><i>Punica</i><i> </i><i>granatum</i><i>, </i><i>Vitex</i><i> </i><i>negundo</i><i>, </i><i>Cirtrus</i><i> maxima. </i>AgNPs of plant extracts were prepared using silver nitrate with the respective plant extract. Then they were characterized by FTIR analysis. The respective functional groups in the synthesized silver nanoparticles were confirmed with FTIR Spectra. The antibacterial activities of the synthesized nanoparticle extract were observed by zone of inhibition. From the results, the nanoparticles synthesized from the plants extract could pave a way to formulate a drug to treat microbial infection.

In this Research work, Silver Nanoparticles were green synthesized from Psidium guajava leaves<b> </b>and different Characterization techniques including UV-Visible, FT-IR, SEM and XRD were all employed to ascertain the absorption peaks, functional group, surface morphology and crystalline size of the nanoparticles respectively. These nanoparticles green synthesized were applied against four different pathogens namely, S. aureus (gram- positive bacteria), E. coli (gram- negative bacteria), C. albicans (gram- positive fungus) and Aspergillus niger and the investigation showed that the Silver nanoparticles synthesized were potent against the selected pathogens. From the UV-Vis spectral analysis, it was observed that highest absorption peaks appeared at 400nm and 500nm reflecting the surface plasmon resonance of silver Nanoparticles from guava leaves which is characteristic of Silver Nanoparticles. From the FT-IR spectrum of the sample under studied, the peaks 3416.85 cm^-1, 2923.51 cm^-1, 1618.95 cm^-1, 1384.49 cm^-1 and 1033.63 cm^-1 were observed where the absorption band at 3416.85 cm^-1corresponds to the stretching due to N-H, while the band at 2923.51 cm^-1 is associated with C-H stretch of alkane and O-H stretching. The peak at 1618.95 cm^-1 shows C=C stretching, 1384.49 cm^-1reveals the existence of C-H bending and 1033.63 cm^-1 depicted C-O stretching. The SEM analysis revealed the shape of the nanoparticles as being spherical while XRD result admits that the average size of the green synthesized Ag NPs was 45.5 nm using the Scherer’s formula. Augmentin was used as control at concentration of 300μg/L throughout antimicrobial studies. Different concentrations of 100, 200, 300, 400 and 500μg/L of Silver Nanoparticles were tested against each pathogen. It was discovered that with increase in concentrations of Silver Nanoparticles of all the pathogens, there generally appeared to be increase in inhibition zone. At higher concentration of 500μg/L, the zones of inhibition were in the following order; 22.50 mm, 17.00mm, 15.44mm, and 13.23mm for E. Coli, S. aureus, C. albicans and Aspergillus niger respectively. For each concentration investigated, E. coli, demonstrated higher zone of inhibition as opposed to all other pathogens investigated in this research.