Abstract
In Central Africa, mushrooms are critically important non-timber forest products (NTFPs), both nutritionally and economically. A strain of edible and medicinal lignicolous fungus, Pleurotus tuber-regium (Rumph. ex Fr.) Singer 1951 (strain 190212), isolated from tissue (sclerotia), on PDA medium, was tested on corn grain and sawdust seedling substrates and on palm oil male inflorescence
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In Central Africa, mushrooms are critically important non-timber forest products (NTFPs), both nutritionally and economically. A strain of edible and medicinal lignicolous fungus,
Pleurotus tuber-regium (Rumph. ex Fr.) Singer 1951 (strain 190212), isolated from tissue (sclerotia), on PDA medium, was tested on corn grain and sawdust seedling substrates and on palm oil male inflorescence (
Elaeis guineensis Jacq.), ground corn (
Zea mays L) stalks and grass (
Paspalum notatum L) soaked for 24 hrs then drained for 24 hours, and unsoaked ground corn (
Zea mays L) stalks. The highest mycelial growth rate recorded was about 0.9 cm on the PDA medium; 5.97 cm on the corn-based seedling medium and 11.95 cm on the sawdust-based seedling medium. Total mycelial invasion on the PDA medium was observed on day 10, day 14 on the corn-based seedling medium, and day 24 on the sawdust-based seedling medium. The onset of mycelial invasion was noticeable on day 3 of seeding for all treatments T
0 (control), T
1 (Final substrate based on soaked ground corn stalks), T
2 (Final substrate based on unsoaked ground corn stalks), and T
3 (Final substrate based on turf). Total invasion of mycelium was obtained at day 15 of incubation for treatments T
1 and T
2, at day 18 for treatment T3 and at day 24 for treatment T0. The results obtained on treatments T
1 and T
2 respectively (14.95±3.12% and 15.65±1.06%) of the maize stalk substrate, lead us to believe that the strain 190212 of
Pleurotus tuber-regium species used has adapted and requires an improvement of the medium with nitrogen-rich additives such as soybean meal. This could achieve the theoretical yield of 20% or more, according to which a substrate can be considered better in producing sporophores.
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