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Analysis of D- and L- Isomers of (Meth)amphetamine in Human K2EDTA Plasma
Journal of Biomedical and Life Sciences
| Vol 3, Issue 1
Table 2. Inletsettings for human blood plasma D- and L- assay.
| Inlet Settings | D- and L- Assay |
| Analytical Column | Supelco Astek Chirobiotic V 250 x 2.1 mm, 5 µm |
| Guard Cartridge | None |
| Sample Temperature | 15 ± 5.0°C |
| Column Temperature | 30.0 ± 5.0°C |
| Mobile Phase A | Water:Acetic Acid:Ammonium Hydroxide: Methanol 5:1:0.3:993.5 |
| Mobile Phase B | N/A |
| Needle Rinse | Water:Acetic Acid:Ammonium Hydroxide: Methanol 5:1:0.3:993.5 |
| Flow Rate | 0.3 mL/min |
| Injection Volume | 10 μL |
| Run Time | 11 min |