The study sought to examine the socio-economic and cultural factors that affect women’s participation in local level governance in selected districts in the Central Region of Ghana. The qualitative approach was employed for this study, and an interview guide was used to gather data from 11 women assembly members and two of their husbands, making a total of 13 participants. The participants were sampled using purposive, snowball and convenient sampling techniques. The data was analysed thematically. The study identified lack of self-confidence, limited funds, unsupportive family, abuse and harassment, political affiliations in the Assembly and unsupportive religious organizations as the socio-economic and cultural barriers to women participation in local level governance in Agona West, Gomoa West and Gomoa Central Districts in the Central Region of Ghana. The findings showed that despite the barriers, some of the women were able to surmount such challenges and became successful in the District Assembly elections. The study recommended that special fund should be set up by the government to support Assembly women to perform their roles and responsibilities within their communities regularly. Such funds may help them fulfil their campaign promises, which may encourage them to seek re-election in the District Assemblies. Also, female politicians should mentor potential female politicians. The mentors may support them to build the confidence required to engage in local level politics and to aspire higher positions in political office.