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Quantifying 64 drugs, illicit substances, and D- and L- isomers in human oral fluid with liquid-liquid extraction
Open Journal of Medical Sciences
| Vol 3, Issue 1
Table 9. Gradient for separation of 63 ESpositive analytes consisting of a series of linear step gradients over 8 min.
| Time (minutes) | Flow (mL/min) | % MPA | % MPB | Curve |
| 0 | 0.7 | 93 | 7 | Linear |
| 0.1 | 0.7 | 93 | 7 | Linear |
| 3 | 0.7 | 60 | 40 | Linear |
| 4.5 | 0.7 | 10 | 90 | Linear |
| 6 | 0.7 | 10 | 90 | Linear |
| 6.1 | 0.7 | 93 | 7 | Linear |
| 7.6 | 0.7 | 93 | 7 | Linear |
| 8.02 | Stop |
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Note: electrospray (ES); mobile phase A (MPA); mobile phase B (MPB)